Background: A mixture of three plants was used in this study: Tribulus terrestris (TT), Phoenix dactylifera (Pd) and Nasturtium officinale (No) which were believed to have fertility enhancing effects. Objectives: To study the effects of the aqueous and ethanolic extracts of the mixture of the three plants on body weight and some reproductive organs parameters in male mice. Material and Methods: Extracts of the mixture of the three plants were injected intraperitoneally at doses of 75, 150 and 300mg/kg/day to male mice for a period of one and two weeks. Body weight and reproductive organs weight were recorded. The height and diameter of the epithelial lining cells of the epididymis and seminiferous tubules were evaluated by the end of the two periods through histological preparations. Result: Both extracts showed a significant increase in body weight and reproductive organs weight as well as the diameter and height of the epithelial lining cells of the seminiferous tubules and epididymis. Conclusion: A dose dependent pattern of effects was noticed in both extracts with more obvious effects resulted by the ethanolic extract.
Background: Cadmium (Cd) is a heavy metal toxicant, present widely in our environment and workplaces.It is well known that cadmium causes adverse effects on male reproductive organs and reproductive functions in experimental animals. Objective: This experiment was designed to find out the effect of cadmium on some histological and hormonal parameters of the reproductive system of adult male rats. Methods: thirty mature albino male rat were used (Rattus rattus norvegicus albinus), as a mammalian model. these animals were divided into two groups, 15 rat / group after labeling them. The control group (G1) was orally administrated with distilled water during the 4 weeks period of the experiment and the experimental group (G2) was orally administrated with 20 ml cadmium chloride (200 mg/1L body weight) during the 4 weeks. First of all, the rat were anesthetized, after that blood aspiration using anesthetic cardiac puncture and the serum was separated and store at -20°C until assessment of FSH, LH and testosterone hormones. After that, the weight of reproductive organs (testis,epididymus and seminal vesicle) was recorded. These organs were fixed with 10% formal saline for histological study. Results: The statistical analysis shows a significant (P< 0.05) decrease in the weight of the testes and seminal vesicles when compared with control group. The results of the sperm parameters show a significant (P< 0.05) decrease in sperm concentration and a highly significant (p≤0.01) decrease in sperm motility in treated group as compared to control. The result of testicular sections in treated group shows a decrease in thickness of germ cells layer, widening of the central seminiferous tubules lumen and prominent germ cells population necrosis. Multiple vacuoles were seen within the tubules. Sertoli cells were abnormal in number and shape as compared to control. A peritubular fibrotic change had been seen also in the testicular sections. The result also shows a highly significant decrease in diameters of seminiferous tubules, primary spermatocytes, secondary spermatocytes, spermatids and increase in interstitial space when treated with cadmium compared with control group. The Levels of serum hormones shows a significant (P<0.05) decrease in the levels of testosterone and LH hormones mean in the treated group,while highly significant (ps0.01) increase in the FSH hormone mean in the treated group when compared to the levels mean of the hormone in the control group.. Conclusion: In conclusion, these findings suggest that cadmium administration with 20 ml cadmium chloride (200 mg/1L body weight) for 4 weeks during the adulthood induced damage in the reproductive organs and lead to impair the functions of sperm parameters and cause histological and hormonal alterations.
Lead is a heavy metal toxicant, present widely in the environment and workplaces. Zinc has been shown to reduce the toxicity of lead. The present experimental animal study was designed to observe the changes in the testes due to excessive use of lead. This study aims to detect the effect of antioxidants (zinc) on the toxicity of high doses of lead on the reproductive organs of male mice embryos compared to the control group as a model for human representative. Thirty mature female mice were used (Swiss-Webster), as a mammalian model. They were divided into three groups, 10 mice/group after labelling them. They were randomly assigned into three experimental groups, and these embryos were processed for histological observations. The recorded results showed a severe disruption in the organization of testicular structure with primordial germ cells severely degenerated with the absence of basal lamina and peritubular myoid cells of the treated group (G1) compared with the control group at day 14 and day 17. The embryo testes of (G2) at day 14 showed irregular testes cords with prominent degenerative changes compared to that of the control group (G3). It was concluded from these results that zinc sulphate plays a significant role in the improvement of histological damage of the testes belonging to mothers injected with lead acetate and protects the reproductive organs from injury during lead toxicity.
Background: Tribulus terrestris (TT), Phoenix dactilyfera (Pd) and Nasturtium officinale (No) were believed to have fertility-enhancing effects and given by herbalists in Baghdad to men who need to increase their fertility capacity. Objectives: To investigate the possibility that aqueous and ethanolic extracts of the plants mixture can counteract the adverse effect of theobromine on reproductive system of male mic. Materials and Methods: One hundred and twenty mature male mice were injected intraperitoneally with 250 mg/kg/day of theobromine for four weeks. Then the males were treated intraperitonially with different dose levels of aqueous and ethanolic extracts of a mixture of the three plants for four weeks to evaluate their effects on body weight, reproductive organs weight, sperm parameters, hormones level and reproductive performance. Results: Both extracts showed a significant enhancement in all parameters studied. Almost all these results showed a dose dependent pattem with the ethanolic extract being the more effective than aqueous extract in all parameters. Conclusions: Mixture of the three plants seems to counteract the action of theobromine on the reproductive system of male mice through enhancing the concentration and motility of spermatozoa, increasing epididymis and seminal vesicles weight which are an indicator of testosterone production which brought about the stimulation of all male reproductive organs.
Monosodium glutamate (MSG) is a widely used flavor enhancer in many foods. However, excessive consumption during pregnancy may adversely affect embryonic development and reproductive functions. This study aimed to investigate the effects of maternal MSG consumption during pregnancy on pregnancy outcomes and the reproductive system of female offspring in mice. Thirty adult pregnant female mice, aged 42–49 days, were used in this study. The animals were equally divided into two groups: an experimental group (G1) and a control group (G2). The experimental group received MSG extract orally at a dose of 1 mg/kg body weight dissolved in 1 ml of distilled water (D.W.) throughout the three weeks of pregnancy, whereas the control group received 1 ml of distilled water alone for the same duration. After delivery, litter size and litter weight were recorded. Forty days after birth, the weights of the female reproductive organs were measured, and the diameters of the ovaries and oviducts were assessed. In addition, blood samples were collected for the evaluation of follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol (E2) levels in both groups. The experimental group showed a significant reduction in litter size and a significant decrease in litter weight compared with the control group. Female offspring born to MSG- treated mothers also exhibited a significant decrease in reproductive organ weight, ovarian diameter, and oviduct diameter. Furthermore, significant reductions in the levels of FSH, LH, and estradiol were observed in the experimental group. Maternal consumption of MSG during pregnancy may negatively affect pregnancy outcomes and impair the development and function of the reproductive system in female offspring.
The toxic effect of drugs and environmental chemicals on the human reproductive system has become a major health concern. Lead is recognized as the most dangerous of the toxic metals and a major threat to the biological systems of both human and animals. In this study we detect the effect of two types of antioxidants: flaxseed and zinc, on the toxicity of low dose of lead acetate on reproductive organs of male mice, this study was conduct. Two groups of mature male mice,exposed to low dose of lead, were administered either zinc sulphate (0. 5mg/kg body weight/day) or flaxseed oil (2.5% of total daily food intake), for 6 weeks. The results showed that lead caused a significant reduction in the total body weight, testicular weights & adverse effects on sperm parameters, while the concomitant administration of antioxidants caused elevation in these weights with significant improvement in sperm quality as compared to the only lead treated group. It is concluded that lead, like all other heavy metals, could induce oxidative stress and the concomitant administration of antioxidants (zinc or flaxseed) can ameliorate the toxic effect of lead on reproduction.