Cover
Vol. 14 No. 2 (2024)

Published: December 31, 2024

Pages: 17-38

Research Article

The protective influence of Zinc Sulphate on some histological changes in Male mice embryos belonging to the mother injected with lead acetate.

Nisreen Flayyih 1 Ban Th. Saeed Rana A. Al-Saadi Zahraa Alzaidi
1High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University, Baghdad, Iraq.
History
  • Received: October 7, 2024
  • Accepted: October 30, 2024
  • Available Online: December 8, 2024
DOI

https://doi.org/10.28969/IJEIR.v14.i2.r2.24

Abstract

Lead is a heavy metal toxicant, present widely in the environment and workplaces. Zinc has been shown to reduce the toxicity of lead. The present experimental animal study was designed to observe the changes in the testes due to excessive use of lead. This study aims to detect the effect of antioxidants (zinc) on the toxicity of high doses of lead on the reproductive organs of male mice embryos compared to the control group as a model for human representative. Thirty mature female mice were used (Swiss-Webster), as a mammalian model. They were divided into three groups, 10 mice/group after labelling them. They were randomly assigned into three experimental groups, and these embryos were processed for histological observations. The recorded results showed a severe disruption in the organization of testicular structure with primordial germ cells severely degenerated with the absence of basal lamina and peritubular myoid cells of the treated group (G1) compared with the control group at day 14 and day 17. The embryo testes of (G2) at day 14 showed irregular testes cords with prominent degenerative changes compared to that of the control group (G3). It was concluded from these results that zinc sulphate plays a significant role in the improvement of histological damage of the testes belonging to mothers injected with lead acetate and protects the reproductive organs from injury during lead toxicity.

Keywords

References

  1. Altshuler, N. (2003). Children’s environmental exposures. DCHP paper Series on Children’s Health and the Environment. Washington, D.C. USA; US Environmental Protection Agency . protected the reproductive organs from injury during lead toxicity.
  2. Suk WA, Murray K, Avakian MD. Environmental hazards to children's health in the modern world. Mutat Res. 2003 Nov;544(2-3):235-42. Doi: 10.1016/j.mrrev.2003.06.007. PMID: 14644325.
  3. RÍsovÁ V. The pathway of lead through the mother's body to the child. Interdiscip Toxicol. 2019 Sep;12(1):1-6. doi: 10.2478/intox-2019-0001. Epub 2020 Feb 20. PMID: 32189981; PMCID: PMC7061448.
  4. M. M. El-Tohamy and W. S. El-Nattat, “Effect of Antioxidant on Lead-Induced Oxidative Damage and Reproductive Dysfunction in Male Rabbits,” Journal of American Science, Vol. 6, No. 1, 2010, pp. 613-622.
  5. Abadin H, Ashizawa A, Stevens YW, Llados F, Diamond G, Sage G, Citra M, Quinones A, Bosch SJ, Swarts SG. Toxicological Profile for Lead. Atlanta (GA): Agency for Toxic Substances and Disease Registry (US); 2007 Aug. PMID: 24049859.
  6. Moniem A, Dkhil M, Al-Quraishy S: Protective role of flaxseed oil against lead acetate induced oxidative stress in testes of adult rats. Afr J Biotechnol 2010, 9(42):7216– 7223 .
  7. Singh RP, Shashwat S, Suman K: Free Radicals and Oxidative Stress in Neurodegenerative Diseases: Relevance of Dietary Antioxidants. Journal of Indian Academy of Clinical Medicine JIACM 2004, 5(3):218–225.
  8. Mishra M, Acharya UR: Protective action of vitamins on the spermatogenesis in lead- treated Swiss mice. J Trace Elem Med Biol 2004, 18(2):173–178.
  9. Sebahat T, Bünyamin K, Günfer T, Gülten E and Osman G. Effects of cadmium and zinc on plasma levels of growth hormone, insulin- like growth factor I, and insulin-like growth factor-binding protein 311. Biological Trace Element Research, 2005; 108: 197-204.
  10. Gundacker C, Hengstschläger M. The role of the placenta in fetal exposure to heavy metals. Wien Med Wochenschr. 2012 May;162(9-10):201-6. doi: 10.1007/s10354- 012-0074-3. PMID: 22717874.
  11. Greenpeace (2005). A Chinese child sits amongst a pile of wires and-waste, picture. Pp 53-82.
  12. Alfadda A. A. and Sallam R. M., Reactive oxygen species in health and disease, Journal of Biomedicine and Biotechnology. (2012) 2012, 14, 936486, https://doi.org/10.1155/2012/936486, 2-s2.0- 84866133072.
  13. Venkatesh S, Gurdeep Singh M, Prasad Gupta N, Kumar R, Deecaraman M, Dada R. Correlation of sperm morphology and oxidative stress in infertile men. Iran J Reprod Med. 2009;7:29–34.
  14. Kurpisz, M., Miesel, R., Sanocka, D. and Jendrzejczyk, P. Seminal plasma can be a predictive factor for male infertility. Hum. Reprod., 1996; 11: 1223–1226 .
  15. Henkel, R.R., Defosse, K. and Koyro, H.W. Estmate of oxygen consumption and intercellular zinc concentration of human spermatozoa in relation to motility: Asian, J.Anodrol., 2003; 5:3-8.
  16. Vish wanath, M. Inorganic elements in: Sardesai, Vish wanath, M., Introduction to clinical Nutrition. 2nd Ed. New York, 1998; 98-100.
  17. Klauder DS, Murthy L, Petering HG. Effect of dietary intake of lead acetate on copper metabolism in male rats. In: Hemphill DD, ed. Trace substances in environmental health. Columbia, MO: University of Missouri; 1973:131. 9. Six KM ,
  18. Conrad ME, Barton JC. Factors affecting the absorption and excretion of lead in the rat. Gastroenterology. 1978;74:731±40.
  19. Barltrop D, Khoo HE. The influence of dietary minerals and fat on the absorption of lead. Science Tot Environ. 1976;6:265±73 .
  20. Flora SJS, Jain VK, Behari JR, Tandon SK. Protective role of trace metals in lead intoxication. Toxicol Lett. 1982;13:51±6 .
  21. Kagi JHR, Vallee BL. Metallothionein, a cadmium and zinc-containing protein from the equine renal cortex. J Biol Chem. 1961; 236:2435±42.
  22. Papaioannou RA, Sohler A, Pfeiffer CC. Reduction of blood lead levels in battery workers by zinc and vitamin C. J Orthomol Psychiat. 1978;7:1±13 .
  23. Flora SJS, Singh S, Tondon SK. Thiamine and zinc in prevention or therapy of lead intoxication. J Internat Med Res. 1989;17:68± 75.
  24. Aitken, R.J., Fisher, H.M., Fulton, N., Gomez, E., Knox, W., Lewis, B., and Irvine, S. Reactive oxygen species generation by human spermatozoa is induced by exogenous NADPH and inhibited by the flavor protein inhibitors diphenylene iodonium and quinacrine. Mol Reprod Dev.,1987; 47: 468 - 482.
  25. Corpas, I.; Gaspar, I.; Martinez, S.; Codesal, J.; and Candelas, S. (1995). Testicular alteration in rats due to gestational and early lactation administration of lead. Reprod. Toxicol. 9: 307-313.
  26. Shayne C. Gad, in Encyclopedia of Toxicology (Fourth Edition), 2024.
  27. Homan, C.S. and Brogan, G.X. (1993). Lead Toxicity. In Handbook of Medical Toxicology. Viccellio, P. (ed.). Little, Brown and Company, London. Pp: 271-273.
  28. McGivern, R.F; Sokol, R.Z. and Berman, N.G. (1991). Prenatal lead exposure in the rat during the third week of gestation; Long-term behavioral, physiological, and anatomical effects associated with reproduction. Toxicol. Appl. Pharmacol. 110: 206-215.
  29. Gonzale-Cossio, T.; Peterson, K.E.; Sanin, L.; Fishbein, S.E.; Palazuelos, E.; Aro, A.; Hernandez-Avila, M. and Hu, H. (1997). Decrease in birth weight in relation to maternal bone lead burden. Pediatrics. 100: 856-862 .
  30. (IPCS) (1995). International Panel of Chemical Safety. Inorganic lead- Environmental Health Criteria 165. International Programme on Chemical Safety. World Health Organization, Geneva, Switzerland .
  31. Brent, R.L. (2004). Environmental causes of human congenital malformations, the pediatrician's role in dealing with these complex clinical problems caused by a multiplicity of environmental and genetic factors. Pediatrics. 113(4 suppl). 957-68 .
  32. Danielson, B.R.; Dencker, L. and Lindgren, A. (1983). Transplacental movement of inorganic lead in early and late gestation in the mouse. Arch. Toxicol. 54: 97- 107 .
  33. Odiette, W.O. (1999). Environmental physiology of animals and pollution. Diversified Resources Ltd. Lagos. P 261.
  34. Guyton, A.C. and Hall, J.E. (2016). Textbook of Medical Physiology, Used, Elsevier Saunders, Philadelphia, Pennsylvania. Pp: 1017-1018 .
  35. Heap, R.B.; Flint, A.P.; Hartmann, P.E.; Gadsby, J.E.; Staples, L.D.; Ackland, N. and Hamon, M. (1981). Oestrogen production in early pregnancy. J. Endocrinol. 89 (Suppl). 77 -94 .
  36. Bowman, P. and McLaren, A. (1970). Cleavage rate of mouse embryos in vivo and in vitro. J. Embryol. Exp. Morphol. 24: 203- 207 .
  37. Dey, S.K. (2003). Fertility study shows that increased estrogen shortens the window of implantation in mice. Vanderbilt University Medical Center, [email protected]: 322-4747 .
  38. Tchernitchen, N.N.; Villagra, A. and Tchernitchen, A.N. (1998). Antiestrogenic activity of lead. Environ. Toxicol. Water Qual. 13: 43-53 .
  39. Pinon-Lataillaade, G.; Thoreux-Manlay, A.; Coffigny, H.; Masse, R. and Soufir, J.C. (1995). Reproductive toxicity of chronic lead exposure in male and female mice. Hum. Experiment. Toxicol. 14: 872-878 .
  40. Kostial, K. and Momcilovic, B. (1974). Transport of lead 203 and Calcium 47 from mother to offspring. Arch. Environ. Health. 29: 28-30 .
  41. Silbergeld, E.K. (1983). Experimental studies of lead neurotoxicity. Biol. Reprod. 33: 722-728.
  42. Moorman, W.J.; Skaggs, S.R.; Clarck, J.C. and Simon, S.D. (1998). Male reproductive effects of lead, including species extrapolation for the rabbit model. Reprod. Toxicol. 12: 333-46 .
  43. McGivern, R.F; Sokol, R.Z. and Berman, N.G. (1991). Prenatal lead exposure in the rat during the third week of gestation; Long-term behavioral, physiological, and anatomical effects associated with reproduction. Toxicol. Appl. Pharmacol. 110: 206-215.
  44. Liu, D. Y. and Baker, H. W. G. Tests of human sperm function and fertilization in vitro. Fertil. Steril., 1992; 58:465–483 .
  45. Robert, K. M., Daryl, K.G., Peter, A. M., and Victor, W. K. Harpers Biochemistry.24th Ed.Appleton &Lange, 1997; Pp.155-521.
  46. Aitken, R.J., Fisher, H.M., Fulton, N., Gomez, E., Knox, W., Lewis, B., and Irvine, S. Reactive oxygen species generation by human spermatozoa is induced by exogenous NADPH and inhibited by the flavor protein inhibitors diphenylene iodonium and quinacrine. Mol Reprod Dev., 1987; 47: 468.
  47. Irvine, D.S. Glutathione as a treatment of male infertility. Rev Reprod., 1996; 1: 6-12.
  48. Bernstein, L.; Pike, M.C.; Depue, R.H.; Ross, R.K.; Moore, J.W. and Henderson, B.E. (1988). Maternal hormone levels in early gestation of crypt orchid males: A case- control study. Br.J. Cancer. 58: 379-381 .
  49. Hutson, J.M.; Baker, M.; Terada, M.; Zhou, B. and Paxton, G. (1994). Hormonal control of testicular descent and the cause of cryptorchidisim. Reprod. Fertil. Dev. 6: 151- 156 .
  50. Thoreux-Manlay, A.; Velezdelacalle, J.F.; Olivier, M.F.; Soufir, J.C.; Masse, R. and Pinon-Lataillade, G. (1995). Impairment of testicular endocrine function after lead intoxication in the adult rat. Toxico. 100; 101- 109.