Cover
Vol. 3 No. 2 (2013)

Published: December 31, 2013

Pages: 38-43

Research Article

Effects of Cadmium on sperm parameters, histological and hormonal changes in testes of mature rats.

Ban T. Saeed 1
1Department of Applied Embryology, High Institute For Infertility Diagnosis and Assisted Reproductive Technologies-Al-Nahrain University
History
  • Received: September 1, 2013
  • Available Online: December 1, 2013

Abstract

Background: Cadmium (Cd) is a heavy metal toxicant, present widely in our environment and workplaces.It is well known that cadmium causes adverse effects on male reproductive organs and reproductive functions in experimental animals. Objective: This experiment was designed to find out the effect of cadmium on some histological and hormonal parameters of the reproductive system of adult male rats. Methods: thirty mature albino male rat were used (Rattus rattus norvegicus albinus), as a mammalian model. these animals were divided into two groups, 15 rat / group after labeling them. The control group (G1) was orally administrated with distilled water during the 4 weeks period of the experiment and the experimental group (G2) was orally administrated with 20 ml cadmium chloride (200 mg/1L body weight) during the 4 weeks. First of all, the rat were anesthetized, after that blood aspiration using anesthetic cardiac puncture and the serum was separated and store at -20°C until assessment of FSH, LH and testosterone hormones. After that, the weight of reproductive organs (testis,epididymus and seminal vesicle) was recorded. These organs were fixed with 10% formal saline for histological study. Results: The statistical analysis shows a significant (P< 0.05) decrease in the weight of the testes and seminal vesicles when compared with control group. The results of the sperm parameters show a significant (P< 0.05) decrease in sperm concentration and a highly significant (p≤0.01) decrease in sperm motility in treated group as compared to control. The result of testicular sections in treated group shows a decrease in thickness of germ cells layer, widening of the central seminiferous tubules lumen and prominent germ cells population necrosis. Multiple vacuoles were seen within the tubules. Sertoli cells were abnormal in number and shape as compared to control. A peritubular fibrotic change had been seen also in the testicular sections. The result also shows a highly significant decrease in diameters of seminiferous tubules, primary spermatocytes, secondary spermatocytes, spermatids and increase in interstitial space when treated with cadmium compared with control group. The Levels of serum hormones shows a significant (P<0.05) decrease in the levels of testosterone and LH hormones mean in the treated group,while highly significant (ps0.01) increase in the FSH hormone mean in the treated group when compared to the levels mean of the hormone in the control group.. Conclusion: In conclusion, these findings suggest that cadmium administration with 20 ml cadmium chloride (200 mg/1L body weight) for 4 weeks during the adulthood induced damage in the reproductive organs and lead to impair the functions of sperm parameters and cause histological and hormonal alterations.

Keywords

References

  1. Michael G, Sophie P, Kenneth W, Warren F, Edward Y, Avril M, DanielG and Claude H. Thyroid Toxicity Due to Subchronic Exposure to a Complex Mixture of 16 Organochlorines, Lead, and Cadmium. Toxicol. Sci., 2002; 67 (2): 207-218.
  2. Michael C and Jorge P. Endocrine Disruption by Cadmium, Common Environmental Toxicant with Paradoxical Effects on Reproduction. Exp Biol Med., 2004; 229, 5: 383-392. a
  3. Jung D, Yaping L and Curtis D. Protective effect of metallothionein against the toxicity of cadmium and other metals. Department of Phamacology, Toxicology & Therapeutics, University of Kansas Medical Center, USA.Toxicology., 2001; 163: 93-100.
  4. IARC. Cadmium and cadmium compounds. Beryllium, Cadmium, Mercury, and Exposures in the Glass Manufacturing Industry. Working Group Views and Expert Opinions, Lyon, France. IARC Monograph on Evaluation of Carcinogenis Risks in Human, 1993: 41-117.
  5. Joseph P. Mechanisms of cadmium carcinogenesis.2009; TAAP 238: 272-279.
  6. Anunciación L, Pilar C and Ana I. Are cadmium effects on plasma gonadotropins, prolactin, ACTH, GH and TSH levels, dosedependent? Biometals, 2003; 16:243-250.
  7. Anunciación L, Nuria M, María P, David P and Ana IE. Cadmium Effects on Hypothalamic-Pituitary-Testicular Axis in Male Rats. Exp Biol Med., 2001; 226: 605-611.
  8. Burukoğlu D and Bayçu C. Protective effects of zinc on testes of cadmium-treated rats. Bull. Environ. Contam. Toxicol., 2008; 81:521-524.
  9. Lafuente Aand Esquifino Al. Cadmium effects on hypothalamic activity and pituitary hormone secretion in the male. Toxicology.1999; 110: 209-218.
  10. Sebahat T, Bünyamin K, Günfer T, Gülten E and Osman G. Effects of cadmium and zinc on plasma levels of growth hormone, insulinlike growth factor I, and insulin-like growth factor-binding protein 311. Biological Trace Element Research, 2005; 108: 197204-.
  11. Roser M, Juan M, Victoria C and José L. Dietary Intake of Arsenic, Cadmium, Mercury, and Lead by the Population of Catalonia, Spain. Biological Trace Element Research, 2008; 125: 120-132.
  12. Au D, Reunov A and Wu R. Reproductive impairment of sea urchin upon chronic exposure to cadmium. Part II: Effects on sperm development. Environmental Pollution. 2001; 111: 11-20.
  13. Benoff S, Hauser R, MarmarJ, Hurley I, Napolitano Band Centola G. Cadmium concentrations in blood and seminal plasma: correlations with sperm number and motility in three male populations (infertility patients, artificial insemination donors, and unselected volunteers). In Molecular Medicine, 2009; 15: 248-262.
  14. Toman R,Massanyi P, Hluchy S, Golian J, Pivko J, Lukac M and Jancova A. Ultra structural changes in the testes of rabbits after intraperitoneal and peroral administrations of cadmium. In: Metal lons in Biology and Medicine, 2008; 10: 651-657.
  15. De Souza Predes F, Diamante M and Dolder H.Testis response to low doses of cadmium in Wistar rats. International Journal of Experimental Pathology, 2010; 91: 125-131.
  16. Roychoudhury S, Massanyi P, Bulla J, Choudhury MD, Lukac N, Filpejova T, Trandzik J, Toman Rand Almasiova V. Cadmium toxicity at low concentration on rabbit spermatozoa motility, morphology and membrane integrity in vitro. In Journal of Environmental Science and Health A, 2010; 11: 1374-1383.
  17. Xiaozhong Yu, Sungwoo Hong and Elaine M. Faustman.Cadmiuminduced Activation of Stress Signaling Pathways, Disruption of Ubiquitin-dependent Protein Degradation and Apoptosis in Primary Rat Sertoli Cell-Gonocyte Cocultures..Toxicol. Sci., 2008; 385-396.
  18. Sarah F, Samual B, Roland E and titilayo A.testicular toxicity and sperm quality fallowing cadmium exposure in rat: Ameliorative potentials of allium cepa. Journal of human reproductive science.2012; 37.
  19. Sokol RZ. The hypothalamic-pituitary-gonadal axis as a target for toxicants. Comprehensive Toxicology. Oxford: Elsevier Science, 1997;10:87-98.
  20. Gupta RS, Sharma R, Chaudhary R, Yadav RY and Khan TI. Effect of textile waste water on the spermatogenesis of male albino rats. J. Appl. Toxicol., 2003;23:171-175.
  21. El-Demerdash FM, Yousef MI, Kedwany FS and Baghdadi HH. Cadmium-induced changes in lipid peroxidation, blood hematology, biochemical parameters and semen quality of male rats: protective role of vitamin E and ẞ-carotene. Food Chem. Toxicol., 2004;42:15631571.
  22. Blanco A and Moyano R, Vivo J. Quantitative changes in the testicular structure in mice exposed to low doses of cadmium. Environ. Toxicol. Pharmacol., 2007;23:96-101.
  23. Lafuente A, Esquifino Al. Cadmium effects on hypothalamic activity and pituitary hormone secretion in the male. Toxicol Lett.,2000;110:209-218.
  24. Garside DA and Harvey PW. Endocrine toxicology of the male reproductive system. In: Atterwill CK, Flack JD, editors. Endocrine Toxicology. Cambridge: Cambridge University Press; 1992; 285312.
  25. Li LH and Heindel JJ. Sertoli cell toxicants. In: Korach KS, editor. Reproductive and Developmental Toxicology. New York: Marcel Dekker; 1998; 655-91.
  26. Veldhuis JD, Iranmanesh A, Jhonson ML and Lizarralde G. Twentyfour-hour rhythms in plasma concentrations of adenohypophyseal homones are generated by distinct amplitude and/or frequency modulation of underlying pituitary secretory bursts. J. Clin. Endocrinol., Metab, 1990; 71: 1616-1623.
  27. National Research Council. Guide for the Care and Use of Laboratory Animals. Institute of Laboratory Animal Resources, Commission on Life Sciences, National Research Council, National Academy of Sciences (USA), National Institutes of Health, 1996.
  28. Pasqualotto FF, Locambo CV, Ahayde KS and Arap S. Measuring male infertility: epidemiological aspects. Rev Hosp Clin Fac Med Sao Paulo., 2003;58:173-8.
  29. Lee IP and Dixon RL. Effects of cadmium on spermatogenesis studied by velocity sedimentation cell separation and serial mating. J. Pharmacol. Exptl. Therap.,1973;187: 641.
  30. Ghosh PK, Ghosh AK and Biswas NM Effect of Cadmium on 17-hydroxysteroid Dehydrogenase in total testis. Andrologia, 1987; 19:143-147.
  31. Lafuente A, Márquez N, Piquero S and Esquifino Al. Cadmium affects the episodic luteinizing hormone secretion in male rats: Possible age-dependent effects. Toxicol Lett.,1999; 104:27-33.
  32. Paksy K, Varga B, Horvath E, Tatrai E and Ungvary G. Acute effects of cadmium on preovulatory serum FSH, LH and prolactin levels and on ovulation and ovarian hormone secretion in oestrus rats. Reprod Toxicol., 1989; 3:241-247.
  33. Lafuente A, Marquez N, Perez-Lorenzo M, Pazo D and Esquifino Al. Cadmium effects on hypothalamic-pituitary-testicular axis in male rats. Exp. Biol. Med., 2001; 226: 605.
  34. Nation JR, Frye GD, Von Stultz J and Bratton GR. Effects of combined lead and cadmium exposure: Changes in schedulecontrolled responding and in dopamine, serotonin, and their metabolites. Behav Neuroendocr, 1989; 103:1108-1114.
  35. Gupta A, Gupta A, Murthy RC, Thakur SR, Dubey MP and Chandra SV. Comparative neurotoxicity of cadmium in growing and adult rats after repeated administration. Biochem Int., 1990; 21:97-105.
  36. Das KP, Das PC, Dasgupta S and Dey CC. Serotoninergiccholinergic neurotransmitters> function in brain during cadmium exposure in protein restricted rat. Biol Trace Element Res, 1993; 36:119-127.
  37. De souza predes F, Diamante M. and Dolder H. Testis response to low doses of cadmium in Wistar rats. In International Journal of Experimental Pathology, 2010; 91: 125-131.
  38. Antonio MT, Corpas I and Leret ML. Neurochemical changes in newborn rat>s brain after gestational cadmium and lead exposure. Toxicol Lett., 1999: 104:1-9.
  39. Selgas L, Arce A, Esquifino Al and Cardinali DP. Twenty-four hour rhythms of serum ACTH, prolacitn, growth hormone and thyroid - stimulating hormone and of median eminence norepinephrine, dopamine and serotonin, in rats injected with Freund>s adjuvant. Chronobiol Int., 1997; 14:253-265.
  40. John W, Georgia L, Susan C and Joy F. Reproductive effects of low acute doses of cadmium chloride in adult male rats. Toxicol and Applied Pharmacol., 1984; 73: 250-255.
  41. Coni E, Baldini M, Stacchini P and Zanasi F. Cadmium intake with diet in Italy: A pilot study. J Trace Elem Electrolytes Health Dis, 1992; 6:175-181.
  42. López-Artíguez M, Soria ML, Cameán A and Repetto M. Cadmium in the diet of the local population of Seville (Spain). Bull Environ Contam.Toxicol., 1993; 50:417-424.
  43. Li H and Wu S. Current situation of research on toxicity of cadmium on the female gonad and reproduction. China Public Health, 2002; 18:379-381.
  44. Shirama K, Furuya T, Takeo Y, Shimizu Kand Maekawa A. Influence of pinealectomy on circadian patterns of plasma luteinizing hormone, follicle-stimulating hormone, testosterone and dihydrotestosterone in the male rat. Endocrinol., Invest, 1982; 5, 397-401.