Cover
Vol. 4 No. 1 (2014)

Published: July 31, 2014

Pages: 28-32

Research Article

Effect of Aloe vera extracts on the histological features of male mice testis

Ibtisam Jasim Sodani 1 Zena Muzhir Hussain
1High Institute for Infertility Diagnosis and ART/ Al-Nahrain University, Baghdad, Iraq
History
  • Received: April 1, 2014
  • Available Online: July 1, 2014

Abstract

Background: Among various natural therapeutic remedies, Aloe vera, a medical plant widely used in food supplements, beverages, pharmaceutical and cosmetics. Aloe vera has been widely reported for its numerous medicinal effects. Objectives: The present investigation was carried out to study the histological changes in the mice testis consumed low doses of Aloe vera leaf gel extracts. Methods: Forty immature male Swiss Webster mice divided into two equal groups (experimental and control) (G and C respectively). The experimental group (G) was given 10 µl of Aloe vera extract orally for 21 days. While the control groups (C) were given by the same dose and rout of administration with normal saline only. After Six weeks (around puberty) the male were sacrificed to get their testes; each testis was fixed. Then histological sections with a thickness of 5 microns were prepared. Results: Histological studies of mice testes from groups that consume low dose Aloe vera extracts showed enhanced the entire structure of spermatogenic layer of semineferous tubules that composed of extensive normal arranged germ cells layer. A significant increase in the numbers of spermatogonia, primary spermatocytes and spermatids, rates of normal supporting (Sertoli cells) and Leydig's cells. A significant increase in the rate of height of epithelial cells, and rates of sperms concentration in the semineferous tubules were observed too. Conclusion: Using low dose of Aloe vera extract enhanced the histological structure of male mice testis and may be useful to produce drugs to improve male fertility.

Keywords

References

  1. Grover J, Yadars, Vats V. Medicinal plants of India with anti-diabetic potential. J Ethnopharmacol. 2002; 81 (1): 81-100.
  2. Olowokudejo JD, Kadiri AB, Travih VA. An Ethnobotanical Survey of Herbal Markets and Medicinal Plants in Lagos State of Nigeria Ethnobotanical Leaflets 2008; 12: 85165.
  3. Hu Y, Xu J, Hu, Q. Evaluation of antioxidant potential of Aloe Vera (Aloe barbadensis Miller) extracts. J. Agric. Food Chem. 2003; 51(26):7788-7791.
  4. Kosif R, Akta G, ?ztekin A. Microscopic Examination of Placenta of Rats Prenatally Exposed to Aloе barbadensis: A Preliminary Study. Int J Morphol. 2008; 26 (2): 275-281.
  5. Rodriguez F, Baldassarre H, Simonetti J, Aste F, Ruttle JL. Cervical versus intrauterine insemination of ewes using fresh or frozen semen diluted with Aloe Vera gel. Theriogenology. 1988; 30 (5): 843-54.
  6. Boudreau MD, Beland FA. An Evaluation of the Biological and Toxicological Properties of Aloe Barbadensis (Miller), Aloe Vera. Journal of Environmental Science and Health Part C. 2006; 24: 103-154.
  7. King GK, Yates KM, Greenlee PG, et al. The effect of Acemannan Immunostimulant in combination with surgery and radiation therapy on spontaneous canine and feline fibrosarcomas. Journal of the American Animal Hospital Association. 1995; 31 (5): 439-47.
  8. Eshun K. He Q. Aloe vera: A Valuable Ingredient for the Food, Pharmaceutical and Cosmetic Industries. Critical Reviews in Food Science and Nutrition. 1995; 44 (2): 9196.
  9. Nassiff HA, Fajardo F, Velez F. Effect of Aloe Vera on hiperlipidemia in patient's refractory's diet. Rev Cuba Med Gen Integr. 1993; 9:43-51.
  10. Bottenberg MM, Wall GC, Harvey RL, Habib S. Oral Aloe Vera-induced hepatitis, The Annals of pharmacotherapy. 2007;41 (10): 1740-3.
  11. Langmead L, Feakins RM, Goldthorpe S, et al. Randomized, double-blind, placebo-controlled trial of oral Aloe Vera gel for active ulcerative colitis. Alimentary pharmacology and therapeutics. 2004; 19 (7): 739-47.
  12. Shamim Sumbul, Ahmed S. Waseemuddin, Azhar Iqbal. Antifungal activity of Allium, Aloe, and Solanum species. Pharmaceutical Biology. 2004; 42 (7): 491-498.
  13. Ferro VA, Bradbury F, Cameron P, Shakir E, Rahman SR, Stimson WH. In vitro susceptibilities of Shigella flexneri and Streptococcus pyogenes to inner gel of Aloe barbadensis Miller Antimicrobial agents and chemotherapy 2003; 47 (3): 1137-9.
  14. Kim HS, S Kacew, BM Lee. In vitro in Streptozotocin Induced Diabetic Rats. Libyan chemopreventive effects of plant polysaccharides Agriculture Research Center Journal International, (Aloe barbadensis Miller, Lentinus edodes. 1999; 1(5): 310-312.
  15. Zhang L, IR. Tizard. Activation of an organ as a possible cause of developmental mouse macrophage cell line by acemannan: the abnormalities in diabetic NOD mice. Histology and major carbohydrate fraction from Aloе vera gel. Histopathol. 1996; 4: 343-358.
  16. Zont F, Bogoni P, Masotti P, Micali G. High-performance liquid chromatographic profiles of Aloe vera constituents and determination of aloin in beverages, with reference to the EEC regulation for flavouring substances. J Chromatogr. 1995; 718: 99-106.
  17. lamnishi K, Aloctin A. An active substance of Aloe vera arborescens as an immunomodulator. Phytother Res. 1993;7:20-22.
  18. Peng SY, Horman J, Curtin G, Corrier D, McDaniel HR, Busbee D. Decreased mortality of Norman murine sarcoma in mice treated with the immunomodulator, Acemannan. Mol Biother. 1991;3:79-87.
  19. Jasem E, Nasim J. Spermatogenic activity of Aloe vera in adult male rats Pharmacology online.2011; 2: 886889.
  20. Vogler BK, Ernst E. Aloe vera: A systematic review of its clinical effectiveness. Br J Gen Pract. 1999: 49:823828.
  21. Townsend J. Aloe Vera. The UK reference quide to complimentary medicine. London: Chartwell House Publishing; 1998.
  22. Atherton P. Aloe Vera revisted. Br J Phylotherapy. 1998; 4:176-183.
  23. Hayes SM. Lichen planus-Report of successful treatment with Aloe vera. Gen Dent. 1999;47:268-272.
  24. Yamaguchi I, Mega N, Sanada H. Components of the gel Aloe vera burm. Biosci Biotechnol Biochem. 1993: 57:1350-1352.
  25. Lee KY, Weintraub ST, Yu BP. Isolation and identification of a phenolic antioxidant from Aloe Vera barbadesis. Free Radical Biol Med. 2000: 28:261-265.
  26. Bancroft, J.D. and Steven, A. (1982). Theory and practice of histological techniques. Churchill, Livingston, London, 2nd.ed. Pp110-111.
  27. Boudreau MD, Beland FA (2006). "An Evaluation of the Biological and Toxicological Properties of Aloe Barbadensis (Miller), Aloe vera". Journal of Environmental Science and Health Part C 24: 103-154.
  28. Jafaribarmak M, Khaksar Z. Effect of Aloe vera extracton testicular tissue of embryo of diabetic rats. Armaghan danesh. 2012; 17 (68)): 149-155.
  29. Yuji Ikeno, Gene B, Hubbard, Shuko Lee, Byung Pal Yu1, Jeremiah T. Herlihy. The influence of long-term Aloe vera ingestion on age-related disease in male Fischer rats. Phytotherapy research. Phytother Res. 2002; 16:712718.
  30. Gumieniczek A, Hopkala H. Wojtowich Z, Nikolajuk J. 32 Changes in antioxidant status of heart muscle tissue in experimental diabetes in rabbits. Acta Biochim Pol. 2002; 49: 529-535.
  31. Baynes JW. Role of oxidative stress in development of complications in diabetes. Diabetes. 1991; 40:405-412.
  32. Halliwell B, Gutterridge JM. Lipid peroxidation, oxygen radicals, cell damage and antioxidant therapy. Lancet. 1994; 1:1396-1397.
  33. Polidori MC, Stahl W, Eichler O, Niestroj I, Sies H. Profiles of antioxidants in human plasma. Free Radic Biol Med. 2001; 30: 456-462.
  34. Wohaieb SA, Godin DV. Alterations in free radical tissue defense mechanism in streptozotocin-induced diabetes in rats. Effects of insulin treatment. Diabetes. 1987; 36: 1014-1018.
  35. Badami S, Gupta MK, Suresh B. Antioxidant activity of the ethanolic extract of Striga orobanchioides. J Ethnopharmacol. 2003; 85: 1227-1230.
  36. Braca A, Sortino C, Politi M, Morelli I, Mendez J. Antioxidant activity of flavonoids from Licania licaniaeflora. J Ethnopharmacol. 2002; 79: 1379-1381.
  37. Ro JY, Lee B, Kim JY, Chung Y, Chung MH, Lee SK. Inhibitory mechanism of aloe single component (Alprogen) on mediator release in guinea pig lung mast cells activated with specific antigen-antibody reactions. J Pharmacol Exp Ther. 2000; 292:114-21.
  38. Davis D, Hartman D Ati-inflammatory and wound healing activity of a growth substance in Aloe vera. J Am Pedia Med Assoс. 1994; 84:77-81.
  39. Nwanjo HU. Antioxidant activity of the exudates from Aloe barbadensis leaves in diabetic rats.Biokemistry. 2006; 18(2): 77-81.
  40. Gunnarsson D, Svensson M, Selstam G, Nordberg G. Pronounced induction of testicular PGF (2 alpha) and suppression of testosterone by cadmium-prevention by zinc. Toxicology. 2004; 2:49-58.
  41. Maurice M. Handbook of African Medicinal Plant. 1993: 2:114-115.