Background: Many plants have medical importance due to the presence an active compounds in plants extraction acting as a drugs for treatment of several diseases in human and animals. Euphorbia helioscopia extract was used to treat diabetes, removal the warts of the skin, stimulate the nervous system and also used against constipation. Objective: Study the effect of terpinoid extract of E.helioscopia on rat epididymal sperm activity and fertility efficacy of male rats. Methods: This study was conducted at Biol. Dept., College of Science/ University of Babylon and Medical College, Mahidol University- Thailand. The study was designed to purify the active compounds of the Terpinoid of Euphorbia helioscopia extract by Nuclear Magnetic Resonance (NMR), and studying their effects on sperm motility and grade activity of the epididymal sperms of the rats. Six main active compounds were isolated from the terpinoid extract of E. helioscopia due to the differences in color, weight and nature by using NMR. The main terpinoid compounds were named A,B,C,D,E and F. Two concentration (0.5 mg/ml and 1 mg/ml) of each active compound of E. helioscopia extract were added to sperm suspension in vitro and incubated for 30 minutes, then sperm motility and grade activity were assessed and the results were compared to those values before adding the active compounds (control). Results: The results showed that 0.5 mg/ml of C and D active terpinoid compounds caused significant (p<0.05) decrease in sperm motility compared to before adding the extract, while the same concentration of B,C,D,E and F active terpinoids compound caused a significant (p<0.05) decrease in grade activity. The addition of 1 mg/ml of terpinoid active compounds (C,D and F) caused a significant (p<0.05) decrease in sperm motility, while the grade activity significantly (p<0.05) decreased by adding B,C,D,E and F active terpinoid compounds compared to control. The sub - compounds (C1,C2,D1,D2,D3 and D4) had a powerful decreasing effect on the sperm motility and grade activity compared to control and other active compounds. Conclusion: It was concluded that terpinoid compounds of E. helioscopia extract have adverse effects on rat epididymal sperm activity.
Background: Tannery work share exposure to solvents and chemicals , which has been linked with inverse pregnancy and fertility outcomes in human being as reported in some studies . Objective: The current study aimed to through some light on the harmful pollutant chemicals effect on tannery workers reproductive health. Literature in this portion are rare in Iraq, so it had been tested the hypotheses that leather workers suffered from male infertility parameters due to the continuous exposure to chemicals that used in this industry. Subjects, Materials and Methods: This study included 60 subjects can divided into 2 groups: 30 males as study group and 30 males as control group, the period of study was between October 2014 to April 2016, the samples were taken from tanneries workers from various governorates of Iraq. Results: The data clearly showed a significant infertility parameters among these workers represented by low total sperm number, low sperm activity the significance levels were within the value of P ≤0.05 accompanied with a significant high seminal fructose. Conclusion: The main conclusion was that chronic exposure to these chemicals in tannery workers especially Cr VI and N,N-dimethyle form amide may cause male infertility parameters.
Background: The increasing prevalence of infertility cases is becoming a major public health problem in developing countries due to changes in diet and lifestyle. Objectives: Black Carrot Extract BCE (Daucuscarota) plays an important role as fertility agent due to its antioxidant and anti-proliferative properties. Therefore, the aim of the present study was to identify the effects of different concentrations of BCE on the outcome of in vitro human sperm activation of infertile patients. Methods: Thirty two semen samples were prepared which served as a control, using direct swim-up technique. Modified Earl's medium (MEM) was used only in group 2 (G2) and two concentrations of BCE (10µg/1ml and 20µg/1ml) were added to culture media MEM of groups 3 (G3) and 4 (G4) for in vitro sperm activation (ISA). Results: A highly significant (P<0.001) correlation was noticed between G4 with G2 and G3 in progressive motility when activation by adding 20µg/1ml of BCE to culture media as compared to G3 (which adding 10µg/1ml of BCE to culture media) and G2 (activation with culture media only). Moreover, swim-up activation technique with culture media and by both adding (10µg/1ml and 20µg/1ml of BCE respectively) to culture media enhancement in the (sperm motility, progressive sperm activity and sperm morphology). Conclusion: The high concentration of BCE is effective in the enhancement of progressive sperm activity during in vitro human sperm activation.