The research objective was to evaluate the blood vitamin B12 levels in females before and after two months of vitamin B12 supplementation. Additionally, the study intended to investigate the effects of this supplementation on antral follicle count (AFC), the number of dominant follicles (DF), the outcome of intrauterine insemination (IUI), and the effect of the outcome by age, and BMI, cause of infertility and types of infertility . A randomized controlled trial was undertaken, including 100 couples that have provided comprehensive data. Segregated into two equivalent groups based on their vitamin B12 intake. Groups A and B, group. Group A comprises 50 pairs of individuals using B12 supplements for eight weeks, and Group B contains no additional elements. Collect blood and seminal plasma samples from all couples to assess their vitamin B12 levels. The two groups underwent ovulation induction and intrauterine insemination (IUI) concurrently with the medical evaluation. Group A started after two months of supplement, but Group B began immediately. Vitamin B12 increases female blood levels, antral follicle count, and dominant follicles (DF). The pregnancy rate has little effect. The antral follicle count and numbers of dominant follicles did not differ between pregnant and non-pregnant women after vitamin B12 supplementation.
Male infertility is a global health issue affecting over 20 million males. A spermatogenesis-related cell differentiation abnormality causes teratozoospermia (TZS). Extensive research has linked TZS to poor fertilization outcomes at assisted reproductive technology (ART) centers as well as to spontaneous pregnancies. This study used Glycyrrhiza glabra (Gg) and Platelet Rich Plasma (PRP) media to establish a culture medium that aimed to improve certain sperm functions of teratozoospermic men in vitro. A total of fifty semen samples were examined. The World Health Organization 2021 and 1999 standards were used to evaluate certain sperm parameters and the DNA fragmentation index. Platelet-rich plasma and Gg extract were utilized as a medium for in vitro preparation and activation of semen samples complaining of teratozoospermia factor. Following activation by the utilization of the density gradient approach and the combination of Gg and PRP medium, there was a statistically significant ( p <0.001) improvement in certain sperm function metrics. These improvements included a higher percentage of morphologically normal sperm, a rise in active motility, and a drop in the DNA fragmentation index. The density gradient technique, regardless of the medium used in this investigation, resulted in a significant enhancement of morphologically normal sperms with other certain sperm parameters and a decrease in the DNA fragmentation index.