Cover
Vol. 14 No. 2 (2024)

Published: December 31, 2024

Pages: 1-16

Research Article

The Influence of Density Gradient Technique and Autologous Platelet Rich Plasma Mixed with Glycyrrhiza Glabra Root Extraction Medium on DNA Fragmentation Index of Teratozoospermic Men

Noor Kadhim 1 Saad S. Al-Dujaily Laith A. Al-Anbari 2
1High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University, Baghdad, Iraq.
2College of Medicine, Al-Nahrain University, Baghdad, Iraq
History
  • Received: October 7, 2024
  • Accepted: October 30, 2024
  • Available Online: December 8, 2024
DOI

https://doi.org/10.28969/IJEIR.v14.i2.r1.24

Abstract

Male infertility is a global health issue affecting over 20 million males. A spermatogenesis-related cell differentiation abnormality causes teratozoospermia (TZS). Extensive research has linked TZS to poor fertilization outcomes at assisted reproductive technology (ART) centers as well as to spontaneous pregnancies. This study used Glycyrrhiza glabra (Gg) and Platelet Rich Plasma (PRP) media to establish a culture medium that aimed to improve certain sperm functions of teratozoospermic men in vitro. A total of fifty semen samples were examined. The World Health Organization 2021 and 1999 standards were used to evaluate certain sperm parameters and the DNA fragmentation index. Platelet-rich plasma and Gg extract were utilized as a medium for in vitro preparation and activation of semen samples complaining of teratozoospermia factor. Following activation by the utilization of the density gradient approach and the combination of Gg and PRP medium, there was a statistically significant ( p <0.001) improvement in certain sperm function metrics. These improvements included a higher percentage of morphologically normal sperm, a rise in active motility, and a drop in the DNA fragmentation index. The density gradient technique, regardless of the medium used in this investigation, resulted in a significant enhancement of morphologically normal sperms with other certain sperm parameters and a decrease in the DNA fragmentation index.

Keywords

References

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