Cover
Vol. 3 No. 2 (2013)

Published: December 31, 2013

Pages: 49-53

Research Article

Morphometric study of kidney and suprarenal gland development after super ovulation injection in mice

Mohammad Oda Selman 1 Ban Thabit Al-Ani Rana R. Al-Saadi Usama I. M. AL-Zubaidi
1Department of Applies Embryology, High Institute for Infertility Diagnosis and Assisted Reproductive Technology, Al-Nahrain University
History
  • Received: September 1, 2013
  • Available Online: December 1, 2013

Abstract

Background: Superovulation in mice causes a delayed embryonic development in vitro and in vivo, an increased abnormal blastocyst formation, pronounced fetal growth retardation. Objective: Morphometric measurements the dimensions (thickness, length) of fetal kidney and suprarenal gland during the later intrauterine life, after superovulation induction. Methods: Twenty five mature female mice Swiss-Webster mice, these mice were divided into two groups: experimental (G1) (15animal/group) and control group(G2) (10 animal/group). Superovulation was induced by intraperitoneal (IP) injection of 7.5 IU of PMSG,then followed by 7.5 IU of HCG after 48 hours. Ovulation was taken place between 122± hours after injection of HCG. One mature male mice was placed with three superovulated female mice for mating. Pregnant females were sacrificed on gestational day eighteen in all experimental groups. For morphometric study 20 mice embryos were fixed in Bouin>s fixative, longitudinal sections of whole embedded embryo were stained by H&E, the specimens independently read. The dimensions (thickness, length) of fetal kidney and suprarenal gland were evaluated. Results: The statistical analysis showed significant decrease (P<0.01) in weights of fetuses in comparison with control group. The study was showed significant decrease (P< 0.01) in fetal kidney dimensions at day 18 of intrauterine life, when it compared to the fetal kidney of non superovulated group(control group). There was also significant decrease (P< 0.01) in the morphometric measurements of fetal suprarenal gland dimensions. Conclusion: The study improve that there is a delay in growth of kidney and suprarenal gland in fetal mice that belongs to mother undergo to superovulation induction before pregnancy.

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