Cover
Vol. 9 No. 1 (2019)

Published: November 30, 2019

Pages: 111-134

Research Article

Comparing the Activation Role of Centrifugation Swim-up and the Density Gradient Combined with ALLGrad Sperm Preparation Techniques

Sahar Muhssin Muhammad 1 Muhammad-Baqir Muhammad-Rashad Fakhrildin 2
1High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al- Nahrain University, Baghdad, Iraq
2Jaber Bin Hayyan Medical University, Baghdad, Iraq
History
  • Received: May 16, 2019
  • Accepted: December 5, 2019
  • Available Online: December 11, 2019
DOI

https://doi.org/10.28969/IJEIR.v9.i1.r7

Abstract

In about 40% of infertile couples, the husband was found to be either the single or a contributing cause of infertility and the systemic management ready for those couples with male infertility are restricted. 135 couples with infertility shared herein; the husbands required in vitro activation for their sperm samples, their wives however; had no fertility problem and were submitted ovarian induction protocol. However; Sperm preparation was done using both, the centrifugation indirect swim-up technique with Global medium and density gradient with ALLGrad solution. While ALLGrad 45% is a ready-to-be-used upper layer. There was an acceleration in seminal parameters after preparation using both various techniques. So, a highly significant increment (P≤0.001) in the percentages of progressive forward motility was in grades (A and B) and grade (A) progressive sperm motility, also a significant enhancement (P≤0.05) in percentage of morphologically normal sperms when applying density gradient mechanism in relation to centrifugation swim-up technique. This study concludes that the use of density gradient mechanism with ALLGrad solution and Global culture medium are more efficient in achieving better percentages of progressive forward motility grades (A and B), grade (A) progressive motility and morphologically normal values of spermatozoa than centrifugation indirect swim-up technique by using Global culture medium. However, it was found there was a non-significant difference when we use both techniques concerning other sperm parameters.

Keywords

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