Cover
Vol. 8 No. 2 (2018)

Published: December 31, 2018

Pages: 50-59

Research Article

Effect of Exposure to Progesterone During Intrauterine Period on Reproductive System of Female Mouse Morphological and Biochemical Study

Hanaa A. F. Al-Sadee 1 Insaf J. Al-Hassun 2 Nawal Kh. Al-Ani 3
1High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al- Nahrain University, Baghdad- IRAQ
2Al-Kindy College of Medicine-Baghdad University,Baghdad-IRAQ
3College of Pharmacy-Al- Nahrain University, Baghdad- IRAQ
History
  • Received: September 1, 2018
  • Available Online: December 1, 2018
DOI

https://doi.org/10.28969/IJEIR.v8.i2.r8

Abstract

Background : progesterone used as a therapeutic agent in human pregnancy. The sufficient evidence to hypothesize that progesterone may have teratogeniceffect on the reproductive system of female offspring. Objectives: This study was established to explore the individual impact of differ­ ent doses of progesterone during intrauterine period on reproductive system of female mouse, and on some reproductive parameters. Materials and Methods: ninety adult female mice (8-10 weeks) and weight (25-28) gm divided into three groups. Control group (G1) was treated daily (IM) injection with sesame seed oilfrom 1-14 day of pregnancy, and other two groups that treated with different doses of progesterone G2 (0.4mg/kg. B.wt/day), G3 (0.8mg/kg. B.wt/day), daily (IM) injection of progesterone from 1-14 day of pregnancy then selecting of Female offspring and sacrificed at age zero day, one month, two month. Parameter analysis involvedanogenital distance , body and ovary weights, , length of epithelial cells, number and diameter of follicles (Primordial , Primary , Secondary and Mature antral) , corpus luteum and using histological section by (motic image plus) and hormonal assay including proges­ terone and Estrogen. Results: The results of this study demonstrated that at one month, two months after birth, there was a highly significant increase P< 0.01 in theanogenital dis­ tance . The statistical analysisshowed no statistical significant difference in the body and ovary weights , at one month after birth, The statistical analysisshowed highly statistically significant decreased(P< 0.01 ) in both G2 and G3 in the number of follicles . At one,two months after birth, there was a highly significant decreased (P< 0.01 ) in both G2 and G3 in the number ofcorpora lutea. At age two month, there was a highly significant decreased (P< 0.01 ) in both G2 and G3 in the height of epithelial cell layer.There was a highly significant decreased (P< 0.01 ) in both G2 and G3 in the diameter of follicles (Primordial , Primary , Secondary and Mature antral) follicles . There was a highly significant decreased (P< 0.01 ) in both G2 and G3 in Estrogen and Progesterone levelof female off­ spring when compared with the control group G1. Conclusion: The results showed higher doses of progesterone for long period in pregnancy can impact some reproductive parameters of female offspring mice. Keywords : Progesterone, corpora lutea, Estrogen,anogenital distance

Keywords

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