Cover
Vol. 8 No. 2 (2018)

Published: December 31, 2018

Pages: 36-42

Research Article

Association of Seminal Plasma Level of NLRP3 Inflammasome Protein with Quality of Seminal Fluid Parameters Among In­ fertile Men

Nuha A. Younis 1 Haider F. Ghazi 2 Ula M. AL-Kawaz Hussain Kh. Kadhem 3
1High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al- Nahrain University, Baghdad- IRAQ
2High College of Medicine, Al- Nahrain University, Baghdad- IRAQ
3Diyala Health Directorate, Al Batool Teaching Hospital , Department of Infertility, Diyala-IRAQ
History
  • Received: September 1, 2018
  • Available Online: December 1, 2018
DOI

https://doi.org/10.28969/IJEIR.v8.i2.r6

Abstract

Background : inflammasome protein govern the inflammatory process. The ele­ vation of NLRP3 inflammasome might be related to bad seminal fluid analysis results. Objectives: This study aimed to evaluate seminal plasma NLRP3 protein and its relation with seminal fluid analysis parameters before and after in vitro sperm activation. Patients and Methods: a total of 50 infertile patients were collected form higher institute of infertility diagnosis and assisted reproductive technique, there se­ men samples were processed for seminal fluid analysis before and after in vitro sperm activation. NLRP3 inflammasome protein were measured in seminal plas­ ma. Results: the mean level of NLRP3 protein was 5.12 ng/ml. the higher level of NLRP3 protein (4.5ng/ml) associated with lower sperm concentration, grade A, B, C and higher level of grade D motility. However, 50% of those patients have an improvement in their seminal fluid analysis after in vitro sperm activation. Conclusion: Elevation of NLRP3 protein in seminal plasma may be associated with the increased leukocytospermia, bad sperm quality and unresponsiveness to in vitro sperm activation among infertile male.

Keywords

References

  1. Elliott EI, Sutterwala FS. Initiation and perpetuation of NLRP3 inflam­ masome activation and assembly. Im­ munol Rev. 2015;265(1):35-52.
  2. Guo H, Callaway JB, Ting JP-Y. Inflammasomes: mechanism of ac­ tion, role in disease, and therapeutics. Nat Med. 2015;21(7):677-687.
  3. Ibrahim E, Zhang X, Aballa T, et al. INFLAMMASOME INACTIVATION IMPROVES SPERM MOTILITY IN MEN WITH SPINAL CORD INJU­ RY. JURO. 2013;189(4):e773.
  4. Jurewicz M, Ibrahim E, Attia G, Roberge S, Lynne C, Brackett N. In­ flammasome activation in men with abnormal semen quality. Fertil Steril. 2014;102(3):e9.
  5. Travis A, Cardona C, Moody M, Simpson A, Ostermeier GC. INDI­ CATORS OF INFLAMMASOME ACTIVATION IN MEN WITH AB­ NORMAL SEMEN PARAMETERS. J Urol. 2016;195(4):e909.
  6. Hayrabedyan S, Todorova K, Jabeen A, et al. Sertoli cells have a functional NALP3 inflammasome that can mod­ ulate autophagy and cytokine produc­ IJEIR ISSN: 2616-6984 tion. Sci Rep. 2016;6(1):18896.
  7. Omu AE, Al-Qattan F, Al-Abdul- Hadi FM, Fatinikun MT, Fernandes S. Seminal immune response in infertile men with leukocytospermia: Effect on antioxidant activity. Eur J Obstet Gy­ necol Reprod Biol. 1999;86(2):195- 202.
  8. Fraczek M, Sanocka D, Kamieniczna M, Kurpisz M. Proinflammatory cy­ tokines as an intermediate factor en­ hancing lipid sperm membrane per­ oxidation in in vitro conditions. J Androl. 2008;29(1):85-92.
  9. Fraczek M, Szumala-Kakol A, Dworacki G, Sanocka D, Kurpisz M. In vitro reconstruction of inflammato­ ry reaction in human semen: Effect on sperm DNA fragmentation. J Reprod Immunol. 2013;100(1):76-85.
  10. Jiang L, Zheng T, Huang J, et al. Association of semen cytokines with reactive oxygen species and his­ tone transition abnormalities. J As­ sist Reprod Genet. 2016;33(9):1239- 1246.
  11. Shekarriz M, Sharma RK, Thom­ as AJ, Agarwal A. Positive myelop­ eroxidase staining (Endtz test) as an indicator of excessive reactive ox­ ygen species formation in semen. J Assist Reprod Genet. 1995;12(2):70- 74.
  12. Matthews DE, Farewell VT. Using and Understanding Medical Statis­ tics. S. Karger AG; 2015. Iraqi Journal Of Embryos and Infertility Researches Vol.(8) Issue (2) (2018) IJEIR ISSN: 2616-6984
  13. La Vignera S, Vicari E, Condorel­ li RA, D’Agata R, Calogero AE. Male accessory gland infection and sperm parameters (review). Int J Androl. 2011;34(5 PART 2).
  14. La Vignera S, Condorelli RA, Vicari E, et al. Markers of semen inflamma­ tion: Supplementary semen analysis? J Reprod Immunol. 2013;100(1):2- 10.
  15. Azenabor A, Ekun AO, Akinloye O. Impact of inflammation on male reproductive tract. J Reprod Infertil. 2015;16(3):123-129.
  16. Atig F, Raffa M, Habib BA, Kerkeni A, Saad A, Ajina M. Impact of seminal trace element and glutathione levels on semen quality of Tunisian infertile men. BMC Urol. 2012;12:2-9. Iraqi Journal Of Embryos and Infertility Researches Vol.(8) Issue (2) (2018)
  17. Havrylyuk A, Chopyak V, Boyko Y, Kril I, Kurpisz M. Cytokines in the blood and semen of infertile patients. Cent Eur J Immunol. 2015;40(3):337- 344.
  18. Fettelschoss A, Kistowska M, Lei­ bundGut-Landmann S, et al. Inflam­ masome activation and IL-1 target IL-1 for secretion as opposed to sur­ face expression. Proc Natl Acad Sci. 2011;108(44):18055-18060.
  19. Martinon F, Burns K, Tschopp J. The inflammasome: a molecular plat­ form triggering activation of inflam­ matory caspases and processing of proIL-β. Mol Cell. 2002;10(2):417- 426.
  20. Inoue M, Williams KL, Gunn MD, Shinohara ML. NLRP3 inflammasome induces chemotactic immune cell mi­ gration to the CNS in experimental autoimmune encephalomyelitis. Proc Natl Acad Sci. 2012;109(26):10480- 10485.
  21. Abal M. Characterizing the con­ tribution of inflammasome-derived exosomes in the activation of the immune response. Ann Transl Med. 2017;5(7):172-172.
  22. Minutoli L, Antonuccio P, Irrera N, et al. NLRP3 Inflammasome In­ volvement in the Organ Damage and Impaired Spermatogenesis Induced by Testicular Ischemia and Reperfu­ sion in Mice. J Pharmacol Exp Ther . 2015;355(3):370-380.
  23. Miao EA, Rajan J V, Aderem A. Caspase‐1‐induced pyroptotic cell death. Immunol Rev. 2011;243(1):206- 214.
  24. Feldmann J, Prieur A-M, Quartier P, et al. Chronic Infantile Neurologi­ cal Cutaneous and Articular Syndrome Is Caused by Mutations in CIAS1, a Gene Highly Expressed in Polymor­ phonuclear Cells and Chondrocytes. Am J Hum Genet. 2002;71(1):198- 203.
  25. Guarda G, Zenger M, Yazdi AS, et al. Differential Expression of NLRP3 among Hematopoietic Cells. J Immu­ nol. 2011;186(4):2529-2534.
  26. Ibrahim E, Castle SM, Aballa TC, et al. Neutralization of ASC im­ proves sperm motility in men with spinal cord injury. Hum Reprod. 2014;29(11):2368-2373.