Cover
Vol. 8 No. 1 (2018)

Published: June 30, 2018

Pages: 38-45

Research Article

Advanced Stimulatory Method for Activation of Sperm Func-tion Parameters by using TAD Glutathione in Iraqi Infertile Men

Azher S. Hindal 1 Hayder A.L.Mossa Muayad S.Abood
1High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al-Nahrain University, Baghdad-IRAQ
History
  • Received: March 1, 2018
  • Available Online: June 1, 2018

Abstract

Background: One of the major concerns among the male reproductive health is the inability to achieve parenthood even after regular unprotected intercourse. The efficiency of the sperm selection techniques is expressed as the concen-tration of spermatozoa with normal motility. Discontinuous density gradient centrifugation (DGC) method widely used over the last decade due to effec-tiveness and reproducibility in recovering most motile sperm, which separates sperm based on their motility, size, and density differential. Glutathione act as an amino acid donor during spermatogenesis. If the levels of glutathione are too low during spermatogenesis, the number of mature and morphologically normal spermatozoa generated will be decreased. Objectives:The aim of this study was to assess the beneficial role of TAD 600mg Glutathione combined with DGC technique for activation of human sperm of asthenozoospermic, oligozoospermic and normozoospermic subjects compared with the DGC technique alone. Patients, Materials and Methods:Sixty males were involved in this study, di-vided into three groups, (twenty asthenozoospermic, Twenty oligozoospermic and twenty normozoospermic subjects)during their attendance to the Infertility Clinic at High Institute for Infertility Diagnosis and Assisted Reproductive Tech-nologies; Al-Nahrain University. Semen samples were obtained, and seminal fluid analysis was assessed. Each semen sample was divided into three parts. The first part prepared as in vitro sperm characterization before activation, the second part using density gradient centrifugation technique, while the last part prepared using density gradient centrifugation combined with TAD 600mg Glu-tathione. Results: After in vitro sperm activation for asthenozoospermic, oligozoospermic and normozoospermic samples a significant increase was observed in the sperm function parameters including sperm concentration, motility and morphological-ly normal sperm percentage as compared to pre-activation using combined tech-niques (density gradient centrifugation and TAD 600mg Glutathione) as com-pared to DGC technique alone. Conclusions:TAD 600mg Glutathione combined to the DGC technique was found to give higher significant results on sperm function parameters (sperm concen-tration, motility and morphology) when using a low quality of semen samples such as decreased sperm motility as compared with DGC technique alone.

Keywords

References

  1. European IVF-Monitoring Consortium (EIM) for the European Society of Hu- man Reproduction and Embryology; Cal- haz-Jorge, C.(August 2016). “Assisted reproductive technology in Europe, 2012: results generated from European registers by ESHRE”. Human reproduction (Ox- ford, England). 31 (8): 1638–1652.
  2. Spalekova E, Makarevich A, and Piv- ko J.Effect of caffeine on parameter of ram sperm motility. Slovak J. Anim. Sci.2011;44(2):78-83.
  3. Teppei T., Yasushi Y., Shinnosuke K., Takashi K., Hiroji U. & Akira I. Effect of density gradient centrifugation on re- active oxygen species in human semen. , Systems Biology in Reproductive Medi- cine.2017;63:3, 192-198.
  4. petyim S,Choavaratana R, Suksom- pong S, et al. Outcome of sperm prepara- tion Using Double-Gradients Technique Study in Siriraj Hospital. J Med A ssoc Thai.2009;92(7): 878-884.
  5. Luberda,Z. (2005). The role of gluta- thione in mammalian gametes .Reprod Biol, 5: 5-17.
  6. Pahune, P.P., Choudhari, A.R. and Muley, P.A. (2013) The total antioxi- dant power of semen and its correlation with the fertility potential of human male subjects. J Clin Diagn Res.2013;7:991– 995.
  7. De-Matos,D.G. and Fumus,C.C. The importance of having high glutathione (GSH) level after bovin in vitro matu- ration on embrio development effect of β-mercaptoethanol, cysteine and cysteine. Theriogenology;2000;53: 761-771.
  8. Astarto N W, Tjahyadi D, and Jatnikasari S. Comparison between two-layer density gradient and three-layer density gradient technique for sperm preparation at aster fertility clinic, Dr. Hasan Sadik in Gen- eral Hospital.IJIHS.2014;2(1):40-4.
  9. Ghorbani M, Vatannejad A, and Kho- dadadi I. Protective Effects of Glutathi- one Supplementation Against Oxida- tive Stress During Cryopreservation of Human. US National Library of Medi- cine.2012; 37(1): 34-40.
  10. Camargo L, Viana J.H, Sa W.F, Fer- reira A, Ramos A, and Filho V. Factors influencing in vitro embryo produc- tion. Animal Production.2006; 3(1): 19- 28.
  11. Suarez S, and Ho H. Hyperactivat- ed motility in sperm. Reproduction in Domestic Animals. 2003; 38(2):119- 124.
  12. Astarto N W, Tjahyadi D, and Jatni- kasari S. Comparison between two-layer density gradient and three-layer density gradient technique for sperm prepara- tion at aster fertility clinic, Dr. Hasan Sadikin General Hospital. Internation- al Journal of Integrated Health Scienc- es.2014;2(1):40-4.
  13. Ali E, Al-Kawaz U, and Fakhrildin MB. A new sperm preparation technique by combination of density gradient cen- trifugation and glass wool filtration tech- nique versus each one alone for infertile men with asthenozoospermia. Interna- tional Journal of Advance Research.2016; 4(4):1112-1115.
  14. Shams Alddin N.N, Al-Dujaily S.S, and Al-Sultani YK. In vitro sperm activa- tion with pentoxifylline and L-carnitine for infertile men semen usinglayering and sedimentation techniques. Iraqi Jour- nal of Embryos and Infertility Research- es.2013;3(6): 32-37.
  15. Malvezzi H, Sharma R, Agarwal A, Abuzenadah A, and Abu-Elmagd M. Sperm quality after density gradient cen- trifugation with three commercially avail- able media: A controlled trial. Reproduc- tive Biology and Endocrinology.2014; 12:121.
  16. Naher, Z. et.al. (2011). Role of glu- tathione in male infertility. Bangladesh Journal of Medical Biochemistry.2011; 4(2):20-25.
  17. Lundin K, Soderlund B, and Ham- burger L. The relationship between sperm morphology and rates of fertilization, pregnancy and spontaneous abortion in an in-vitro fertilization / intracytoplasmic sperm injection programme. Human re- production.1997; 12(12):2676-81.
  18. Karabulut A, and Tekin A. Alteration in the morphology and motility of sper- matozoa:relation with total sperm count. Pam Med J.2013; 6(1): 1-4.
  19. Bur R, Siegberg R, Mathews C, and Flaherty S. The influence of spermatozoa morphology and the number of motile spermatozoa inseminated on the outcome of intrauterine insemination combined with mild ovarian stimulation. Fertil Steril.1996; 65(1):127-132.
  20. Jayaraman, Upadhya D, Narayan PK, and Adiga SK. Sperm processing by swim-up and density gradient is effective in elimination of sperm with DNA dam- age. J Assist Reprod Genet.2012; 29(6): 557–563.
  21. Naam A. Hamza , Mohammed O. Sel- man, Hayder A. L. Mossa. Comparison of Best Yield of in vitro Sperm activation Techniques with New technique of Caf- feine Combined with Density Gradient Centrifugation in Iraqi Patients. J. Pharm. Sci. & Res.2018;10(1):36-39. Iraqi Journal Of Embryos and Infertility Researches Vol.(8) Issue-1 (2018)