Cover
Vol. 3 No. 2 (2013)

Published: December 31, 2013

Pages: 2-10

Research Article

Relationship between antiMüllerian ovarian hormone, activin-A, and follistatin hormones levels with pregnancy rate following intrauterine insemination

Saad S. Al-Dujaily 1 Nidhal Salim Alwan
1Clinical Reproductive Physiology, High Institute for Infertility Diagnosis and Assisted Reproductive Technologies, Al-Nahrain University, Baghdad-Iraq
History
  • Received: September 1, 2013
  • Available Online: December 1, 2013

Abstract

Background: Recently the reproductive hormones, Anti-Mullerian hormone (AMH), activin A, and follistatin (FS) have been found to play an important role in folliculogenesis, oocyte maturation and corpus luteum function by changing the pattern of granulosa cell expression which in turn affects the success of fertilization potential. Objectives: To examine the relation of in vivo concentrations of AMH, activin A, and FS hormones on the ovaries status in the phases of menstrual cycle and to elucidate the relationship of these hormones with pregnancy rate following intrauterine insemination(IUI). Materials and methods: Seventy seven infertile couples were recruited from infertility clinic population at the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies through the period from September 2011 to May 2012. Those infertile couples were divided to 3 groups according to the infertility cause (male factors causes, anovulatory causes and unexplained causes of infertility). Measurements of AMH, activin A, and FS hormones levels were done at cycle day (CD25-). Then measurements of activin A, and FS hormones levels were done only at preovulatory cycle(CD1214-) when the size of the Graffian follicle range between 1824-mm and at least one follicle was present and before hCG injection and at day 28 of the cycle (CD28). IUI was performed 3640- hours after hCG injection then measurement of the three hormones was performed after successful IUI. The result of successful pregnancy was recorded and statistical relation with AMH, activin A and FS hormones was assessed. Results: There was a positive significant correlation between endometrial thickness (ET) and pregnancy rate and the best ET to predict pregnancy is more than 7mm. There was no significant relation (P>0.05) between AMH level and pregnancy rate. A significant correlation was found between follistatin levels in preovulatory (CD 1214-) with a cutoff value 0.20ng/ml to predict pregnancy. There was a significant relation between activin-A level and pregnancy rate with a predictive cut off value 397.5 ng/ml on (CD 28) only. Conclusions: Activin-A can be regarded as a biomarker candidate for diagnosing very early pregnancy at luteal phase following IUI. Follistatin preovulatory (average CD13) has the most predictive value for pregnancy following IUI.

Keywords

References

  1. drugh W, and Lipshultz LI, Male factu nfeltlity Endccnnd Metab 2004; 88
  2. Brek RP, Krant N, Bakkum et al. Ciferent surgcal techni(pes to reduce adhesion formation, A systematlc review and meta.anatysis. Hum Reprcd 2012 19
  3. Dunson DB, Bard DD and Cocobo B Increased infenilitywith age in men andwanen. J Obst Gynecå 2004
  4. Netsu S, Konno R, and Odagiri K Possble therapy infertility Fedil 2008; 907 502-1496
  5. Altmae S A, J, et al. in fdiate pathway Fes are assmiatedwith unexplained female infertility, Fedil Seil. 2010; 94 130-137.
  6. Agarwal A, Gupta S and Sharma RK Rde oxidative stress in female reprochcticn Hum Revol 200$ 3: 28
  7. Bliss SP, Navratil AM, Xie J, gal. GnRH sigul'ng, thegmadotrcpe and endoyine ütrd of fertility.
  8. Wcrid Health (Ygamzaticn: Laborat«y Manual the examination of human semen and semen-cervical mucus interacticn 4th ed. UK_ Cambridge Univers'tyPress, 1999
  9. Malikawi HY, Hussai S and Qublan PS The effect Of metfTmin rlus clomiphene citrate m mulation and cyratesin clcrr.phene resistant women with pdycystic ovary symdrcrne_ Saud Med J 2002; 23.663-666.
  10. Jun SHI Lathi RB and Wkstphal LM. The roles ot human-derived and recombinant tolcle.sti-nulat.ng hormme in assisted reprcdlctö,'e techndogy_ US Cdst&Gyne 200.7; 42.
  11. AbCujaitySS,AJ.Nakash AR and A-Biaty sm Effectofpentüifyline on the outcome of artificial inseminatim, Irae Postgrachate Med J,20W
  12. Harris l. S and Hcrnstein, u Poa success Of gaudctropininduced cmtrolled ovarian hyperstinulaticn and intrauterine insemination older wanen. Fertil Steril 2010, 94 144—148.
  13. Glover Tand Mitchel K. ntrcOuctimt0Bi$tatistics. 2ndedWaveland press USA 2008, Pp4
  14. Al-Dujaily SS and Aburgheef MA Corre ation between in vitro sperm preparaticn technicpes, endcnetrial thickness, hurr,Mal profile and successful premancy rate fdlmvig IUI: retrcspect've and p ospective study Emb Res.
  15. A-Cujaily SS, Al-Janae AS and Ncri M. Effed o' Gl•ycyrrh'za enract on in vtro Sperm activat'm Of asthenozocspermic patients. J. Babylm Uni
  16. Vrmtikis A, Chang P, Kovacs P, etal Antralfdlide ccwnts (AFC)preåct ovarian response and preglancy cutccmes in "yte donatim cycles J Reprod Genet. 2010' 27t7): 383-389.
  17. La Marca Broekmans FJ, Fauser BC, et al. Anti-W erian hcrmcne (AIIH): what dowe still need to know? Hum. Reprcd 2009; 24 2275.
  18. KharyRH, Wang QF andCruteyWF_ Serum 181 Birdsall M, Ledger W, Nand G, et al. Inhibin A and admin A in the frst trirnester of human pregnancy. J
  19. Ro$laei ME Sabet F and Mohamadi K accuracy ofserum activin A in detectim ot ectopic preglancy J Res Med Sci 2012: 17(4): 378-381.
  20. JH, MS, Gwang Y, et al. Serum biomarkers fu predcting pregnancy cutcane in wornen undergdng IVF human chcnulic gmadotrcvn, progesterme, and inhibin A level at 11 days post.ET. Clin ReptCd 2012,
  21. Roghaei MA, Sabet F and "/chamadi K Diagnostic accuracy ot serum activin A detectim megnanCy. J Res Med SC' 2012; 17 81.