Cover
Vol. 3 No. 1 (2013)

Published: July 31, 2013

Pages: 44-48

Research Article

Relationship between potency of hypo osmotic swelling test and antisperm antibody assay for fertile and infertile men in Thi-Qar governorate

Basim K. Gootee 1
1Department of Biology -College of Science- Thi-Qar University, Thi-Qar-Iraq
History
  • Received: April 1, 2013
  • Available Online: July 1, 2013

Abstract

Background: The sperm plasma membrane activity and absence of antisperm antibodies in infertile patients ejaculate significantly associated with fertilization potential of human spermatozoa. The sperm viability detected by hypoosmotic swelling test (HOST) and autoimmune infertility detected by antisperm antibody assay presented diagnostic and prognostic tools for male infertility factors. Objective: This study was devised to compare the results of hypoosmotic swelling test and antisperm antibody assay for normozoospermic men and infertile patients affected by autoimmune infertility. Methods: Thirty semen samples were collected by masturbation and prepared by direct layering technique. Hypo-osmotic swelling test was performed by mixing 0.1 ml of semen with 0.9 ml of a 150 mOsm/L NaCl as a hypo-osmotic solution and direct immunobead assay were used to determine the presence of Antisperm Antibodies (ASA) bound on sperm surface. However, sperm concentration, sperm motility, and normal sperm morphology were evaluated according to World Health Organization (WHO) criteria and subjected to HOS test and ASA assay. The sperm prepared and incubated for 30 minute in 5% CO2 at 37°C after in vitro sperm preparation. Results: The results of the present study reported that the percentage of HOS test score in antisperm antibodies positive sperm samples (52.30 ±5.2) was significantly lower than that noticed in antisperm antibodies negative (70.30 ± 3.4) sperm samples. Conclusion: It was concluded that the use of HOS test as a simple and diagnostic and prognostic tools for identify male infertility. Further studies are suggested to assess the effect of ASA on sperm plasma functional integrity after ICSI and in vitro fertilization and embryo transfers (IVF-ET).

Keywords

References

  1. Jeyendran RS, Van Der Ven HH, Perez-Pelaez M, Crabo BG and Zaneveld LJD. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil. 1984; 70: 219-228.
  2. Kajstura J and Reiss K. Measurement of cell swelling in a hypotonic medium as a rapid and sensitive test of cell injury. Folia. Histochem. Cytobiol. 1989; 27:39-47.
  3. Katsoff D and Check JH. Two methods of achieving pregnancies despite subnormal hypo-osmotic swelling test scores. Fertil. Steril.1997; 68: 549551
  4. Katsoff D, Check ML and Check JH. Evidence that sperm with low hypoosmotic swelling scores cause embryo implantation defects. Arch. Androl. 2000; 44:227-230.
  5. Tartagni M, Schonauer MM and Cicinelli E. Uselfulness of the hypoosmotic swelling test in predicting pregnancy rate and outcome in Couples undergoing intrauterine insemination. J. Androl.2002; 23:498502.
  6. Katsoff D, Check JH. Bollendorf A and Benfer K. Chymotrypsingalactose treatment of sperm with antisperm antibodies results in improved pregnancy rates following in vitro fertilization. Am. J. Reprod. Immunol.1994; 13: 514-527.
  7. Katsoff D, Check ML and Check JH. Evidence that sperm with low hypoosmotic swelling scores cause embryo implantation defects. Arch. Androl. 2000; 44:227-230.
  8. Kiefer D, Check JH and Katsoff D. The value of motile density, strict morphology, and the hypo-osmotic swelling test in in vitro fertilization with embryo transfer. Arch. Androl.1996; 37:57-60.
  9. Liu J, Tasi Y, Katz E, Compton G, Garcia JE and Baramki TA. High fertilization rates obtained after intracytoplasmic sperm injection with 100% non-motile spermatozoa selected by using a simple modified hypoosmotic swelling test. Fertil. Steril.1997; 66:35-37.
  10. OmuAE, Makhseed M, Mohammed AT and Munim RA. Characteristics of men and women with circulating antisperm antibodies in a combined infertility clinic in Kuwait. Arch. Androl.1997; 39: 55-64.
  11. Vantman D, Zinaman M and Sherins R. Dissociation of the hypoosmotic tail swelling patterns in sperm from fertile and infertile men. J. Androl.1997; 8. (Suppl.): 24.
  12. Harriet GA, Check JH. and Marie-Press JC. Determination of the clinical significance of antisperm antibodies using in vivo insemination techniques. Prog. Reprod. Biol. Med.1992; 15:186-193.
  13. Vigano P, Brigante C, Gonfiantini C, and Doldi N, Busacca M.Which is the best test to evaluate the integrity of sperm plasma membrane. Acta. Eur. Fertil. 2000; 21: 231-234.
  14. Ved S, Montag M, Schmutzler A, Prietl G, Haidl G and Van der Ven HH. Pregnancy following intracytoplasmic sperm injection of immotile spermatozoa selected by the hypo-osmotic swelling test: a case report. Andrologia.1997; 29: 241-242.
  15. Tsai YH, Liu J, Jairo GE, Eugene K, Gial C. and Baramki TA. Establishment of an optimal low hypo-osmotic swelling test by examining single spermatozoa in four different low hypo-osmotic solutions, Hum. Reprod. 1997; 12: 1111-1113.
  16. Check ML, Kiefer D, Check JH, Hourani W and Long R. Treatment of sperm with subnormal HOST scores with chymotrypsin/ viable pregnancy after IUI. Arch. Androl. 2002; 48:155-185.
  17. Tang GWK and Ho P. The relationship between human sperm hypoosmotic swelling test, routine semen analysis, and the human sperm zonafree hamster ovum penetration assay. Fertil. Steril. 1985; 44:668-672.
  18. Smikle CB and Turek PJ. Hypo-osmotic swelling can accurately assess the viability of non-motile sperm. Mol. Reprod. Dev.1997; 47:200-203.
  19. Shanis BS, Check JH. Bollendorf A and Lurie D. Stability of the hypoosmotic swelling test over time. Arch. Androl.1992; 29:263-266.
  20. Sliwa L. Usability of the hypo-osmotic swelling «
  21. AhmadiA. and Soon-Chye NG. The single sperm curling test.a modified hypo-osmotic swelling test, as a potential technique for the selection of viable sperm for intracytoplasmic sperm injection. Fertil. Steril. 1992; 68: 346-350.
  22. Jeyendran RS, Van der Ven HH. and Zaneveld LJD. The hypo-osmotic swelling test: an update.Arch. Androl.1992; 29:105-106.
  23. Avery S, Bolton VN and Mason BA. An evaluation of the hypo-osmotic swelling test as a predictor of fertilizing capacity in vitro. Int. J. Androl.1990; 3:93-99.
  24. Barratt CL, Osborn JC, Harrison PE, Monks N, Dumphy BC, Lenton EA and Cooke ID. The hypo-osmotic swelling test and the sperm mucus penetration test in determining fertilization of the human oocyte. Hum, Reprod. 1989. 4:430-434.
  25. Ahmad K and Naz RK. Effects of human antisperm antibodies on development of preimplantation embryos. Arch. Androl. 1996; 29: 9-20.
  26. Bollendorf A. Check JH. Katsoff D and Fedele A. The use of chymotrypsin-galactose to treat spermatozoa bound with antisperm antibodies prior to intrauterine insemination. Hum. Reprod. 1994; 9: 484488.
  27. Rossato1 M, Galeazzi C, Ferigo M and Foresta C. Antisperm antibodies modify plasma membrane functional integrity and inhibit osmosensitive calcium influx in human sperm. Hum. Reprod.2004; 19: 1816-1820.
  28. Darapa MD, Somboon MD, Thaweephong MD, Phattaraphum MD. and Pitak MD. Hypo-osmotic swelling test supplemented with pentoxifylline after preparation by two-layer percollgradient methods. Fertil. Steril.2004; 56:11-2-5.
  29. Engel S and Petzold R. The spermatozoal volume as indicative of the plasma membrane integrity (modification of the hypo-osmotic swelling test). Methods. Andrologia.1994; 26:309-313.
  30. ChantlerE, Check JH and Abraham-Peskir JV. Significance of midpiece vesicles and functional integrity of the membranes of human spermatozoa after osmotic stress. Andrologia.2002; 36: 87.
  31. Check JH, Bollendorf A, Katsoff D and Kozak J. The frequency of antisperm antibodies in the cervical mucus of women with poor postciotal tests and their effect on pregnancy rates. Amer. J. Hum. Reprod. Immune.1994; 32:38-42.
  32. Check JH and Bollendorf A. Effect of antisperm antibodies on postciotal results and effect on intra-uterine insemination on pregnancy outcome. Arch. Androl 1991; 28:25-31,