Cover
Vol. 3 No. 1 (2013)

Published: July 31, 2013

Pages: 9-15

Research Article

Effect of cryopreservation on some sperm parameters of infertile patients

Muhammad-Baqir M-R. Fakhrildin 1 Israa M. Rasheed
1Department of Clinical Reproductive Physiology, High Institute For Infertility Diagnosis and Assisted Reproductive Technologies-Al-Nahrain University
History
  • Received: April 1, 2013
  • Available Online: July 1, 2013

Abstract

Background: All men have the natural desire to have their own biological children. Cryopreservation of male gametes is an important aspect of human fertility preservation. With the advancement in assisted reproductive technology, indication for sperm cryopreservation is expanding. Objectives: To evaluate the effect of cryopreservation by using Sperm Freeze TM kit on some sperm parameters for infertile patients. Materials and methods: Ninety five semen samples were taken from male patients. The seminal fluid analysis was performed according to the standard criteria of WHO (1999). The patients were divided into four groups depending on their sperm concentration and motility: Normozoospermic, oligozoospermic, asthenozoospermic and oligoasthenozoospermic patients. Cryopreservation in liquid nitrogen was done by using Sperm Freeze TM kit for the samples and thawing was performed after three months. Results: In general, the post-thawing study revealed a considerable highly significant (P <0.001) decrease in the sperm parameters which include the means of sperm concentration, sperm motility, progressive motility and normal sperm morphology as compared to that of pre-cryopreservation. Whereas the percentage of immotile sperm was highly significant (p<0.05) increase as compared to pre-cryopreservation in all groups except for oligozoospermic patients in which there was a significant (p<0.05) increase. Conclusions: After semen cryopreservation, all parameters were significantly decreased.

Keywords

References

  1. Practice Committee of the American Society for Reproductive Medicine. Definitions of infertility and recurrent pregnancy loss. Fertil. Steril. 2008; 90:S60.
  2. Hossain A and Nagamani M. Cryopreservation of male gametes. In: Rizk B, Sallam H, Makriagiannakis A. and Garcia-Velasco J. Infertility and assisted reproduction. 1st ed. New York: Cambridge University Press; 2008. p.466-477.
  3. Shehata F. and Chian Ri. Cryopreservation of sperm: an overview. In: Chian Ri and Quinn P. Fertility Cryopreservation. 1st ed. Cambridge University Press. 2010; p. 39-45.
  4. . Mahony M C, Morshedi M, Scott RT and De Villiers A. Role of spermatozoa cryopreservation in assisted reproduction. In: human spermatozoa inassisted reproduction. In: Acosta A, Swanson R, Ackerman S B, Kruger TA F, J. A. Van Zyl and R. Menkveld. Baltimore: Williams & Wilkins Co; 1990. p. 100.
  5. Polge C, Smith A and Parkes A. Revival of spermatozoa after vitrification and dehydration at low temperatures. Nature. 1949:164: 666.
  6. Sherman JK and Bunge R G. Fertilizing capacity of frozen human spermatozoa. Nature.1953; 172: 767.
  7. Sherman J K. Improved methods of preservation of human spermatozoa by freezing and freeze-drying. Fertil. and Steril. 1963; 14: 49-64.
  8. Sheif S, Raviv G and Eisenberg ML. Posthumous sperm retrieval: analysis of time interval to harvest sperm. Hum Reprod. 2006; 21:289303-
  9. World Health Organisation. WHO Laboratory Manual for the Examination and Processing of Human Semen, 5th ed. Geneva: World Health Organization; 2010
  10. Gadea J. Molla M and Selles E. Reduced glutathione content in human sperm is decreased after cryopreservation: Effect of the addition of reduced glutathione to the freezing and thawing extenders. Cryobiology. 2011; 62: 40-46.
  11. Said T M, Gaglani A and Agarwal A. Implication of apoptosis in sperm cryoinjury. Reproductive BioMedicine Online.2010; 21: 456-462.
  12. Martin G. Cagnon N. and Sabido O. Kinetics of occurrence of some features of apoptosis during the cryopreservation process of bovine spermatozoa. Human Reproduction. 2007; 22: 380-388.
  13. Feldschuh J, Brassel J, Durso N and Levine A. Successful sperm storage for 228 years. Fertil. and steril. 2005; 84: 1017.
  14. El-Fakahany H. and Sakkas d. Assisted reproduction using banked sperm. In: Sperm Banking: Theory and Practice. 2nd ed. UK: Cambridge University Press; 2009. p.112.
  15. World Health Organization. WHO laboratory manual for the 4th ed. Cambridge: Cambridge University Press; 1999.
  16. Lewis S. Is sperm evaluation useful in predicting human fertility?. Reprod. 2007; 134: 31-40.
  17. Al-Ani KNGAn in vitro human sperm activation and intra uterine insemination study using Global and Hams F-12 media. High diploma thesis in assisted reproductive techniques. Institute of Embryo Research and Infertility Treatment, University ofAl-Nahrain. 2009.
  18. Muhammad, S.M. A current technique for human sperm preparation using ALLGrad solution in assisted reproductive technologies for infertile couples. High diploma thesis in assisted reproductive technologies. Institute of Embryo Research and Infertility Treatment, Al-Nahrain University. 2011.
  19. Serre V, Pharm D and Robaire B. Paternal age affects fertility and progeny outcome in the brown Norway rat. Fertil. Steril. 1998; 70: 625 -631.
  20. Gangi CR and Nagler M. Clinical evaluation of the subfertile men. In: Infertility and reproductive medicine. Diamond M P,Decherney AН. Philadelphia: WB Sanders; 1992. 299-318.
  21. Ibrahim A. Assessment of Human Sperm Chromatin Structure in Relation to Sperm Parameters and Assisted Reproductive Technologies. Master of Science thesis in Applied Embryology. Institute of Embryo Research and Infertility Treatment, University of Al-Nahrain. 2010.
  22. Sakkas D and Alvarez JG. Sperm DNA fragmentation: mechanisms of origin, impact on reproductive outcome, and analysis. Fert. Steril. 2010; 93: 1027-1036.
  23. Milardi D, Grande G and Sacchini D. Male fertility and reduction in semen parameters: A single tertiary-care center experience. International Journal of Endocrinology. 2012, Article.
  24. Gaur DS, Talekar M and Pathak VP. Effect of cigarette smoking on semen quality of infertile men. Singapore medical journal, 2007; 48: 11923.
  25. Shaaban, MH. An in vitro human sperm activation study: Using Hams F-12 medium and human serum albumin for sperm preparation for infertile patients. High diploma thesis in assisted reproductive techniques. Institute of Embryo Research and Infertility Treatment, University of Al- Nahrain. 2007
  26. Holt WV. Alternative strategies for the long-term preservation of spermatozoa. Reprod. Fertil. Dev. 1997; 9: 309-319.
  27. Centola GM, Raubertas RF and Mattox JH. Cryopreservation of human semen, comparison of cryopreservatives, sources of variability and prediction of post-thaw survival. J Androl. 1992; 13:
  28. 38. 28. Watson RF. Recent developments and concepts in the cryopreservation of spermatozoa and the assessment of their post-thawing function. Reprod. Fertil. Dev. 1995: 7:871-891.
  29. Nijis M and Ombelet W. Cryopreservation of human semen. Hum Fertil. 2001; 158-163.
  30. Anger JT, Gilbert BR and Goldstien M. Cryopreservation of sperm: indication, methods and results. J Urol. 2003; 170: 1079-1084.
  31. Donnelly ET, McClure N and Lewis SE. Cryopreservation of human semen and prepared sperm: effects on motility parameters and DNA integrity. Fertil. Steril. 2002; 76: 892-900.
  32. Dalzell LH, Thompson-Cree MEM and Mc Clure N. Effect of 24-hour incubation after freezing-thawing on DNA fragmentation of testicular sperm from infertile and fertile men. Fertil. Steril. 2003: 79: 1670-1672
  33. 0 ConnelliM, McClure Nand Lewis SEM. The effects ofcryopreservation on sperm morphology, motility and mitochondrial function. Hum. Reprod. 2002; 17: 704-709.
  34. Ozkavukcu S, Erdemli E, Isik A, Derya O, and Karahuseyinoglu S. Effects of cryopreservation on sperm parameters and ultrastructural morphology of human spermatozoa. J Assist Reprod Genet. 2008; 25: 403-411.
  35. Dan Yu, Simon L, and Lewis SEM. The Impact of Sperm Processing and Cryopreservation on Sperm DNA Integrity. In: Sperm chromatin: biological and clinical applications in male Infertility and assisted reproduction. Springer Science+Business media, USA 2011
  36. Kalthur G, Adiga SK and Upadhya D. Effect of cryopreservation on sperm DNA integrity in patients with teratospermia. Fertil. Steril, 2008; 89: 172-173.
  37. Verza JS, Feijo CM and Esleves SC. Resistance of human spermatozoa to cryoinjury in repeated cycles of thaw-refreezing. International Brazilian Journal of Urology. 2009; 35: 581-590.
  38. Borges EJr. Rossi LM, Locambo de Freitas and Guilherme P. Fertilization and pregnancy outcome after intracytoplasmic injection with fresh or cryopreserved ejaculated spermatozoa. Fertil. and Steril. 2007; 87:316-320.