Cover
Vol. 2 No. 2 (2012)

Published: December 31, 2012

Pages: 15-21

Research Article

Cryopreservation of ovarian cortex and vitrification of immature oocyte

Tahani Al-Azawi 1 Safaa Al Hasani
1Reproductive Medicine Unit, University of Schleswig-Holstein at Luebeck, Ratzburger Alle 160, 23538 Luebeck, Germany
History
  • Received: September 1, 2012
  • Available Online: December 1, 2012

Abstract

Background: Unlike sperm production in men which is continuous, women are born with all their eggs and they do not produce any more. Technique to bank eggs would allow girls and women to have the same reproductive options as men when faced with a serious disease and the long term complications of chemotherapies that threatens to destroy their eggs. Objective: The aim of this study is to improve the efficiency of existing ovarian cryopreservation, by following the most recent method named needle immersed vitrification (NIV) beside the slow freezing method. In this review we discussed also the historical description of the methods used, freezing and vitrification of mature and immature oocytes, this include comparison of principles, procedures and results as reported in the literature. Materials and Methods: A total of 285 fragments of human ovarian tissues were isolated and cryopreserved from 36 women aging 26 - 55 years old in the Reproductive Unit, Lubeck-Germany. Ovarian tissue strips of 1-3 mm thick of ovarian cortical biopsies obtained from patients. The collected human ovarian cortex fragments were assigned to two different cryopreservation procedures, slow-freezing and NIV. On the other hand, a total of 80 patients (100 cycles) with polycystic ovaries (PCO) were underwent in vitro maturation (IVM) treatment. The number of inspirated immature oocytes was 536. Vitrification of immature oocytes were carried out. Results: Successful freezing and storing were performed on 285 fragments of ovarian cortex tissue from 36 patients by two cryopreservation protocols as mentioned before but these tissues do not thaw and use yet. Concerning the IVM, the percentage of maturation, fertilization, survival, embryo transfer, and pregnancy rates were 55.2%, 44.5%, 96.2%, 88.1%, and 10.6%, respectively. Conclusion: Recent advances in freezing technology, modifications of conventional protocols used and continuing optimization of vitrification have efficiently improved the method. These results may lead to increase the possibility of preserving fertility by cryopreservation of ovarian tissue. Ovarian tissue banking can offer hope for cancer patients who want to safe guard their fertility against sterilizing chemotherapy and radiotherapy. Immature oocytes can be successfully isolated from the oophorectomy specimen regardless of the day of menstrual cycle, and undergo IVM and cryopreservation.

Keywords

References

  1. Cohen J, Glligan A, Garrisi J Settiæ up an ART Laboratc,y In Texbock cf Asssled Reproductive Laboratory and Clifcal gerspectives. Gardner 0K, k%issmanA, HcwlesC ShohamZ (ais)_ 2001. Martin DunilzLtd_
  2. Koutlaki N, Schopper 8. Marculis G, Ciedrich K. Al-Hasani S. Human oocyte cryopreservation: Past. present and future Reprod Biomed Online. 2036, 13(31- 427435
  3. Cobo A, Donir,go J, Perez S, Crespo J, Remori J, Pellicet A. Wrificatiom an effective new approach tc oocyte banking amdpreserving Mility in cancer patients,
  4. Dcnnez J, Dolmans Demyl± D, Jadoul P, Pirard C, Squiffet Livebirth alter Mhctcpic transplantation cf cryopreserved ovarian tissue. Lartet, 2004:
  5. Morris R. A case of heteroplastic wa.•ian grattrwfållcwed tyy ard lhedelveryd2Lving r&Record. IOS; 798-.
  6. Oriel Y Oafopculcs K. Al-Hasani S. Crytpreservatm of human ovarian tjsst* and oocyte banking An eye to the future. Acab Med J 2DD5: 3.1722-.
  7. Meirc•w D, Nugent D, The effects of radiotherapy and temotherapy on femah reproduction. Hum Reprod Update. 2001; 7:53S43.
  8. Kim SS. Fettlity Preservation in female cancel patients: Current devebpments and future directions Fertil Steril 2006; 85:1-11.
  9. Blatt J. Pregnancy outcome in long-term snivcrs of chihhocd ünce.•. Med. Pediatr 1999, 3329-33.
  10. Partridge AH, Geber S, Peppercorn J, Sampson E, Knudsen K, Laufer M. Web-based survey cl Militv issues in pung women with breast cancer. J Cin 2004 22.4174-83.
  11. Ozaen B. Al-Hassari S. Techniques Ic,• Ovarian Tlssue, Whole Ovary, Oocyte and Embryo Cryweservation. J Reprod Infenil. 2010;
  12. Kim SS. Battaglia Soules MR. Thefutureol humanovarian cryopreservation and transplantation; Fertility beycnd. Fer,il Steril 2001: 75 lug-5s
  13. CA. Cryopreservation Of oocytes from pre•antral follicles. Hum Reprod Jcdate. 2003: g: 119-29.
  14. Mercw D. Lemn J, Eldar•Geva T. Hardan l, Fndma•l E. Zalel Y pregnancy after trans-plantation cryopreserved ovarian lissue in a patient wdh ova-an failure aner chemotherapy. N Ergl J Yed. 353(3): 31821-
  15. H3G TLIa-d T, Pdzg Tal Su RC Ctnti,rg wh cf irnahre f&omed i'? Ito Tijratim a-É cabal
  16. Siber S'. Kæma N, Kuwavama Successful vilification methcd for ovarian tissue: minimizing from cuarian Iransplanlation. Proceeding of the 24th Eshte Annual Mætirg 2008 Jul 69-.
  17. S. van H. M, Krebs D. Cryopreservatim of human oocytes Hum Reprcd.1987 2 (8): 695-7C•0.
  18. ceLucaR. Santi". Feweli A, Bartclctti T, Coticchio G, Scawveli G. MulticeNer obsevalicrel study on s,tw.cccling cocyte cryopresevation: clinical outcome. Fertil Steril_ 2010. 941562-1558.
  19. Elnahas A, Alcolak E, Abu Marat E, Elnahas T, Elnahas K. Pala*as V. Vilification of human cccytes and different development stages of embryos 152-9.
  20. Fasano G. Moffa F. Dechene J. Englert Y. Demeestete I, Vitrification of in vitm matured cocytes collected Item antral follicles at the tine of marian tissue cryopreservabom Repro. BicL 2011; g:1S0.
  21. Gosden NG. Baird DT. Wade 'C. Webb R. Restoraion of Értlity to oophcrætomisedsheepby ovarian autographs stored at. I EC Hum Reprod_ 1994. 597-603
  22. Chen H, Vin H, Kim SS, Tan SC Gosden RG. Falilityaterintactcvary transplantation. Nature, 2002, 415: 385
  23. AlmcCin CG, Vinguett-Camara Vt, Meister H, Ferreira JO, Franco RL, Cavalcante AR Recovery 01 Mility after grafti,-yg trycpceseNed tissue in rabbits radiotherapy Reprc•d. 2C04; 19:1287-93
  24. Goldziehet J.W and Green JA The colycystic ovary Clinical and histologtal features. J. Ch Erdouiml. Metab. 1962, 22 325-338.
  25. MacDcugall Tan S.L. Balen A. A cr.trolled study comparing patients with and without golyytic ovaries urdergcing II-vitro fertilization. Hum. 1993; 8:233-23
  26. SonWY, Chu,-gJT, Dean N, Denirtas E, Holzer H, ElizurS, Chian RC and Tan SL. of optimal day for cocyte retrieval based the diameter of lhe dominant fclli&e in hCC.primed in vitro maturation cycles Human 2880-2685.
  27. Tror.sc.n A.O. C. art KauscheA In-vitro müation and fertilization and developmental competence of occytes recovered from untreated peycystic patients Fertil Steril.1994 62:353-362
  28. Almahbobi G, Ander—sz C, Hutchinson P. Functional integrity of granulose cells from lhe polycystic avaries Clin_ Endocrin.19% 44:571-580
  29. Barnes FL, KauscheA, TigliasJ Production of embryos fff in wire-matured Fertil Steril_1996
  30. Cha KY and Chian R.' Maturation jn immature human oocytes clinical use Hum Reprod Update.199B; 4:103-120
  31. Hyun CS, Cha son WY, Yoon SA, Kim KA, Lim JK optimal ICSI timing after the first polar body extnsion in in 'itro matuted human oocytes. Hum 1991-1995
  32. Andersen cy M. AGI LoftA. C Two pregnancies following autotrans$antation of frozen/lhawed ovarian tissue. Hum Reprai
  33. Dem,eestae l, Simon P, Emiliani S, Delbære and Engle" Y. Fenlity prese-vatlon: Sucessful transplantatbn of cryopreserved ovarian tissæ il a yourg patient previously treated Hodgkin's disease. 2C07: 12:14371442.,
  34. Hirna Hiraoka K, Kinutari M, Kinutani K Blastocele collapse by micropipetting Io vitrificato-, gives excellent survival outcomes for hunan day 5 and 6 expanded blastocysts. Hum Reprod. 2034: 19: 2884-288b
  35. Chian RC, Kuwayama M, Tan L. Fiah survival rate of tovire cocytes matured in following Vitrif cation Journal of ReproductOn ard Development2C04; 50:685-695
  36. Chen SO, CL, Chen TH, Lai Lin CW, Nwel direct Vdrifi*lion for crycpreserva'jc-l ovarian tissues increases viability arc pregnancy capability in mte Hun Reprol 2006; 21: 2794-28CC.
  37. G Regulation ovarian deveoprent in priütes: and hyg*es EndocrRev1996; 17:121-155
  38. Chian R. Puckett W. Too L ard Tan SL. Pregnancies resultr,g from in matured occytes retneved from patents potycystit ovary syndrome ater griming with human chorionic pnadotropin Ferli SteriL1g99b; 72:639-642
  39. Chian RC and Tan SL. Maturational anddevelopmentalcompetence of cumulus- free inrnalure hwman oocytes derived from stimulated and intracytoplasmic sperm injection cycles. Reprod 3i0Med Online,2C02; 5: 125—132,
  40. Yan Wang, ZhJn Xiao, Lei Li, Fan ard Sharg.Wei Li Nove needle irnrrersed vitrification: a and convenient method With potential advantages in Mouse and human warian tissue cryopreservation. Human Repraiuclico. 2256-2265
  41. Donnez J, Squifflet J. Jadoul P. Demytk D, Cherco AC. Pregnancy and live biff after autotranplantatic-, of frozen-thawed ovarian tissue in a patient with metastatic disease undergoing chemotherapy and hematopoietic stem transplantation. Fert Stetil, 20111
  42. Meirow D, Levon J, Eldar.Geva T, Hardan l, Fridman E Monitoring the ovaries after autotransplantation of cryopreserved ovarian fissue: endocrine studies. in fertilization cydes, and live biff. Fertil Steril. 2027;
  43. Demeestere l. Simon P. Moffa F. Delbaere A. and Engle" Y. Sinh of a second healthy giri mue than 3 years after cryopreserved ovarian graft. Letter to the Hum 2010; 15901591-,
  44. Ernst E. terghotdt S. Jorgensen JS. Andersen CY_ Th first woman Io give birth to children following transplantation of frozenfthawed ovarian tissue. Hum Reprcd, 2515112801
  45. Silber S, Kagawa N, Kuwapma Y, Gosden R Duration fertiity after fresh and ovary transplantation. steril,
  46. Donnez J, Si&r S, Andersen CY, Demeestere l. Piver P, Veirow D. Pellicel A, Dolmans Chldren born after autotransplantation Of crppreserved ovarian tissue. Areviewof 13 livebirths. Ann Med, 2011b; 114-.
  47. p. C. Agnani G. pet* 'C, Rohrlich PS. TWO Obtained after a new technique of autotrans;iantation of cr•npresened ovarian tissue. Abstract of the 25th Annual Meeting of ESKRE, Amsterdam, the Netherlards 2CC9; 28
  48. Sanchez-Serrano M, Crespo J, Mirat*t V, CoboACl Escribå MJ Twins bom aner trarsplantaliw, of ovarian tissue and cccyte vitrification. Fertil
  49. Reuel A. Laufer N. Meir AB. Lebovich M and Mitrani E, Micro-organ ovarian transplantation enat&s pregnancy: a caserepofl Hum Rep. 2011; 18; 17•,
  50. Roux C. Amiot C, Agrari G. Aubard Y, Rohriich PS. Live birth after ovarian tissue autcgraft in a patient with sickte cell disease reated by allogeneic bone marrow transplantation. Fert] Steril 2010' 93(7): 2413.e159-.
  51. Kagaua N, Silber S, Kuwayama Successful vitrification of bovine and human ovarian tissue Repßductive BioMedici'.e Online. 2009, 24' 568577-_
  52. Kuchenmeister U. Kuwayama M. Successful vitrification of GV stage bovine cocytes by in straw dilution method Theriogenology 1997; 47: 169
  53. Bleyer WA The impact of childhood carcer on the United States and the a cancer "nal for clinkians 1 gg040: 355-357
  54. Clxkler Ries LAG, Percy CL. Bunin CR. Intn%uction In: Ries LAG, Smith MA. Gurney JG al. Cancer Incidence ard S'-nival among Chikren and Adolescents 1999. united Slates SEER Program 19751995•. Natonal Cara Institute, SEER Prcgram, Bethesda, MO, LSA
  55. Katancda K, Mamgame TCancer Statists Digest. 'pn J Clin Oncol
  56. Tucker Y, Morton P, Liebermann J Human oocyte wycpreservaticn,: a valid alternative to emboo crnpresævation? European Journa of Obstetics and Gynecdcgy and Reproduäve Bblogy 2004: II 3S S24-S27
  57. Kuleshova L, Lopata A. Vitrificatøn can be more favorable than slow coob-,g Fertility and Sterility. 2002:78: 449—454