Cover
Vol. 14 No. 1 (2024)

Published: June 30, 2024

Pages: 69-77

Research Article

Effects of sperm mitochondrial activity on motility, parameter of human fertility

Alaa Ahmed 1 Thaer M. Farhan 2
1High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University, Baghdad, Iraq.
2College of Medicine, Al-Nahrain University, Baghdad, Iraq
History
  • Received: February 7, 2024
  • Accepted: April 30, 2024
  • Available Online: June 8, 2024
DOI

https://doi.org/10.28969/IJEIR.v14.i1.r6.24

Abstract

Mitochondria play a significant key in sperm metabolism as well as power generation. The mitochondria nucleolus is essential for several sperm activities. such as sperm capping, hyperactivation, and acrosome reactions, and oocyte fusion, mitochondrial membrane potential can be used to study sperm mitochondrial activity, the mitochondrial integrity of sperm is evaluated by examination of mitochondrial membrane potential MMP, which may be achieved by using fluorescent dyes like JC-1 probe is composed of 5,5,6,6-tetrachloro-1,1,3,3-tcarbocyanine iodide. To study the correlation between mitochondrial membrane potential Red MMP and sperm motility . This study included 42 male samples who were separated into two groups. (21 normozoospermic, 21 asthenozoospermia) through the period of attendance at the infertility clinic at High Institute for Infertility Diagnosis and Assisted Reproductive Technologies, Al-Nahrain University, and Um Al-Banin Center for Management and in vitro fertilization the collected semen was obtained and seminal fluid analysis was assisted according to (WHO,2021). fluorescent staining of the sample prepared is done by using an MMP Assay Kit (with JC-1) was performed. A positive significant correlation between sperm motility percentage and red MMP intensity (p < 0.001). Mitochondrial activity plays an essential function in sperm motility and male fertility Male infertility, Sperm Motility, Mitochondrial Membrane Potential, MMP Assay Kit (with JC-1).

References

  1. World Health Organization (WHO) “Infertility” 2010 .
  2. Leisegang, K. “Chapter 2.5 - Malnutrition and Obesity”. In: “Oxidants, Antioxidants and Impact of the Oxidative Status in Male Reproduction.”Elsevier Inc.Academic Press.2019;117-134.
  3. Barbagallo, F. La Vignera, S., Cannarella, R., Aversa, A., Calogero, AE.and Condorelli, RA. Evaluation of Sperm Mitochondrial Function: A Key Organelle for Sperm Motility. J Clin Med. 2020;(29-9)2 :363.
  4. Turner, R.M.” Moving to the beat: a review of mammalian sperm motility regulation,”Reproduction, Fertility and Development.2005; 18(2): 25-38. [ 5 ] Darszon, A. López-Martínez, P. Acevedo, J.J. Hernández-Cruz, A. and Treviño, C.L. )2006)” T-type Ca2+ channels in sperm function,” Cell calcium.2006; 40(2): 241-252. [ 6 ] Visconti P. E.” Sperm bioenergetics in a nutshell,” Biology of reproduction.2012; 87(3):72.
  5. Storey, B. (2008) “Mammalian sperm metabolism: oxygen and sugar, friend and foe,” The International of developmental biology.2008; 52(5–6): 427–437.
  6. Luconi,M. and Baldi, E.”How do sperm swim? Molecular mechanisms underlying sperm motility,” Cellular and Molecular Biology. (Noisy-le-Grand, France).2003; 49(3):357-369.
  7. Losano, J., Angrimani, D., Ferreira Leite, R., Simões da Silva, B., Barnabe, V., and Nichi, M. Spermatic mitochondria: Role in oxidative homeostasis, sperm function and possible tools for their assessment. Zygote.2018; 26(4): 251-260. [ 10 ] Uribe, P., Villegas, J.V., Boguen, R., Treulen, F., Sánchez, R., Mallmann P., Isachenko, V., Rahimi G. and Isachenko, E., 2017 Use of the fluorescent dye tetramethylrhodamine methyl ester perchlorate for mitochondrial membrane potential assessment in human spermatozoa Andrologia,49(9), e12753.
  8. World Health Organization (WHO) “Infertility” 2010 .
  9. Perry, S. W., Norman, J. P., Barbieri, J., Brown, E. B. and Gelbard, H. A. Mitochondrial membrane potential probes and the proton gradient: a practical usage guide. Biotechniques. 2011; 50 ( 2 :) 98 - 115 .
  10. Sivandzade et. al. Analysis of the Mitochondrial Membrane Potential Using the Cationic JC-1 Dye as a Sensitive Fluorescent Probe, Bio-protocol.2019;9(1): e3128 .
  11. Moscatelli, N., Spagnolo, B., Pisanello, M., Lwmma, E. D., De Vittorio, M., Zara, V., et al. Single-cell-based evaluation of sperm progressive motility via fluorescent assessment of mitochondria membrane potential. Sci. Rep.2017; 7 (1): 17931 .
  12. Briggiler-Marin, C. I., Luque G. M., Gervasi, M. G., Oscoz-Susino, N., Sierra, J. M., Mondillo, C., et al. Human sperm remain motile after a temporary energy restriction but do not undergo capacitation-related events. Front. Cell. Dev. Biol.2021; 9: 777086.