Cover
Vol. 14 No. 1 (2024)

Published: June 30, 2024

Pages: 56-68

Research Article

Evaluation of Plasminogen Activator Inhibitor-1 (PAI-1) Levels in Blood Serum and Follicular Fluid (Predictive Marker) and its Effect on Oocyte Maturity in Iraqi Infertile Women Undergoing ICSI

Samhar F. Alwan 1 Rana A. R. Alsaadi 2 Hayder A. L. Mossa 1
1Al-Elwiya Maternity Teaching Hospital, Baghdad-IRAQ .
2High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University, Baghdad, Iraq.
History
  • Received: February 7, 2024
  • Accepted: April 30, 2024
  • Available Online: June 8, 2024
DOI

https://doi.org/10.28969/IJEIR.v14.i1.r5.24

Abstract

Plasminogen Activator Inhibitor-1 expression in serum and follicular fluid plays a crucial role in regulating fibrinolysis during the pregnancy period by blocking the action of other proteins called plasminogen activators. The aim is to assess the Plasminogen Activator Inhibitor-1 level in blood serum and follicular fluid as a predictive marker of pregnancy. This study included Sixty-nine infertile females undergone ovarian stimulation with antagonist protocol and received recombinant follicle-stimulating hormone, at the cycle day two. The serum and follicular fluid were collected from each female on the day of oocyte retrieval, for PAI estimation using the Elisa kit. The data were analyzed using version 23.0 SPSS and Microsoft Office 2010. The serum PAI-1 levels at the day of oocyte retrievals were significantly lower in positive pregnancy women in comparison with non-pregnant women (p < 0.05), Yet no significant follicular fluid PAI-1 levels were noted in comparison with non-pregnant women. There was a negative significant correlation (p > 0.05) between serum and follicular fluid PAI-1 levels on the day of oocyte retrieval according to oocyte ICSI outcome. The evaluation of serum PAI-1 levels can be used as a promising parameter for the prediction of pregnancy outcomes of ICSI patients. It is assumed that the pregnancy rate was affected by PAI-1 level during ICSI cycles. The concentration of PAI-1 in serum and follicular fluid at the retrieval day is not correlated with the oocyte's maturity.

Keywords

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