Cover
Vol. 13 No. 1 (2023)

Published: May 31, 2023

Pages: 86-94

Research Article

Role of Repeating Testicular Sperm Extraction on Sperm Recovery by Using Combination Enzymatic and Mechanical Methods in Non-obstructive Azoospemic Men.

Amina Al-Tameemi 1 Ali Ibrahim Rahim 2 Ula Al-Kawaz
1High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University, Baghdad, Iraq.
2College of Medicine, University of Kufa, Najaf, Iraq.
History
  • Received: April 7, 2023
  • Accepted: April 30, 2023
  • Available Online: May 8, 2023
DOI

https://doi.org/10.28969/IJEIR.v13.i1.r7.23

Abstract

Azoospermic patients have the option of undergoing in vitro fertilization procedures, specifically intracytoplasmic sperm injection (ICSI), for which sperm is retrieved from the patient's own testicles and then injected directly into the oocyte. The aim of the study: evaluate the role of repeated sperm extraction by enzymatic and mechanical mincing techniques in azoospemic patients. A total of 32 males with non-obstructive azoospermia participated in the research. Testicular sperm extraction (TESE) was performed under spinal or general anesthesia after a comprehensive history, physical examination, and investigations. After that, the biopsies underwent through a series of mincing processes, both mechanical and enzymatic. Patients gave information including age, body mass index and previous biopsy attempts. In both enzymatic and mechanical methods there was no significant higher sperm recovery in patients with history of previous testicular biopsies (66.7% vs. 38.5%; p=0.209) and (66.7% vs. 30.8%; p=0.102) respectively. Patients who had undergone prior testicular biopsies had marginally higher sperm recovery rates using both enzymatic and mechanical techniques

Keywords

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