Cover
Vol. 12 No. 2 (2022)

Published: August 31, 2022

Pages: 30-39

Research Article

Increased Expression of NLRP3 Inflammasome in Placentas of Gestational Hypertension

Alaa Najem 1 Wasan Adnan Abdulhameed Nahla AL-Bakri 2
1High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University, Baghdad, Iraq.
2Department of Biology, College of Education for Pure Science (Ibn Al-Haitham) University of Baghdad, Baghdad, Iraq.
History
  • Received: October 5, 2021
  • Accepted: August 17, 2021
  • Available Online: August 17, 2022
DOI

https://doi.org/10.28969/IJEIR.v12.i2.r3.22

Abstract

Node-like receptor pyrin 3 is a tripartite protein composed of an amino-terminal pyrin domin (PYD), nucleotide binding NACHT domine, and a carboxy-terminal leucine-rich repeat (LRR) domine, and consider an intracellular sensor that detects a broad range signal and resulting in the formation and activation the NLRP3 inflammasome. This complex lead to the release of the inflammatory cytokines IL-1β, IL-18 which is important for host defense against invading pathogen. Modern evidence indicates that the NLRP3 inflammasome is involved in gestational hypertension. This study was conducted on fifty pregnant women and divided into two groups: 25 placentas from normotensive mothers having no hypertension before as the control group and 25 from gestational hypertension women as gestational hypertension group, obtained from the Department of Obstetrics and Gynecology in Almosil El Aam Hospital and Alkhanssa Teaching Hospital, Mosul city, Iraq. The placental tissue samples were collected from the central region and processed for an immunohistochemical technique to t h e expression of NLRP3 in placental tissue. The immunohistochemical study of t h e placenta of the gestational hypertension group showed the localization of NLRP3 protein and staining intensity in trophoblast, stroma, and endothelium villi were high quantification compared with placenta from the control group.

Keywords

References

  1. Shirasuna K, Karasawa T, Takahashi M. Role of the NLRP3 Inflammasome in Preeclampsia. Front Endocrinol (Lausanne). 2020 Feb 25;11:80. [Online article link]
  2. Sutterwala FS, Haasken S, Cassel SL. Mechanism of NLRP3 inflammasome activation. Ann N Y Acad Sci. 2014 Jun;1319 (1):82-95. [Online article link].
  3. Liu D, Zeng X, Li X, Mehta JL, Wang X. Role of NLRP3 inflammasome in the pathogenesis of cardiovascular diseases. Basic Res Cardiol. 2017 Dec 9;113 (1):5. [Online article link]
  4. Kelley N, Jeltema D, Duan Y, He Y. The NLRP3 Inflammasome: An Overview of Mechanisms of Activation and Regulation. Int J Mol Sci. 2019 Jul 6;20 (13):3328. [Online article link]
  5. Yung HW, Colleoni F, Atkinson D, Cook E, Murray AJ, Burton GJ, Charnock-Jones DS. Influence of speed of sample processing on placental energetics and signalling pathways: implications for tissue collection. Placenta. 2014 Feb;35 (2):103-8. [Online article link]
  6. Suvarna KS, Layton C, Bancroft JD (Eds.). Bancroft's theory and practice of histological techniques. 8th ed. Elsevier Health Sciences, 2018. [Online article link]
  7. Stødle GS, Silva GB, Tangerås LH, Gierman LM, Nervik I, Dahlberg UE, Sun C, Aune MH, Thomsen LCV, Bjørge L, Iversen AC. Placental inflammation in pre-eclampsia by Nod-like receptor protein (NLRP)3 inflammasome activation in trophoblasts. Clin Exp Alaa, et al. http://doi.org/10.28969/IJEIR.v12.i2.r3.22 37 Immunol. 2018 Jul;193 (1):84-94. [Online article link]
  8. Pontillo A, Girardelli M, Agostinis C, Masat E, Bulla R, Crovella S. Bacterial LPS differently modulates inflammasome gene expression and IL- 1β secretion in trophoblast cells, decidual stromal cells, and decidual endothelial cells. Reprod Sci. 2013 May;20 (5):563-6. [Online article link]
  9. Tamura K, Ishikawa G, Yoshie M, Ohneda W, Nakai A, Takeshita T, Tachikawa E. Glibenclamide inhibits NLRP3 inflammasome-mediated IL-1β secretion in human trophoblasts. J Pharmacol Sci. 2017 Oct;135 (2):89-95. [Online article link]
  10. C Weel I, Romão-Veiga M, Matias ML, Fioratti EG, Peraçoli JC, Borges VT, Araujo JP Jr, Peraçoli MT. Increased expression of NLRP3 inflammasome in placentas from pregnant women with severe preeclampsia. J Reprod Immunol. 2017 Sep;123:40-47. [Online article link]
  11. Hung TH, Charnock-Jones DS, Skepper JN, Burton GJ. Secretion of tumor necrosis factor-alpha from human placental tissues induced by hypoxia- reoxygenation causes endothelial cell activation in vitro: a potential mediator of the inflammatory response in preeclampsia. Am J Pathol. 2004 Mar;164 (3):1049- 61.[Online article link]
  12. Raghupathy R. Cytokines as key players in the pathophysiology of preeclampsia. Med Princ Pract. 2013;22 Suppl 1 (Suppl 1):8-19. [Online article link]