Cover
Vol. 12 No. 1 (2022)

Published: June 30, 2022

Pages: 86-93

Research Article

Effects of PRP Intratesticular Injection in Non-Obstructive Azoospermic Infertile Men Spermatogenesis and Reproductive Hormonal Levels (FSH and LH).

Abstract

Non-obstructive azoospermia (NOA) is the absence of sperm in infertile men ejaculate as a result of significant spermatogenesis defects or hormonal malfunction. PRP intratesticular injection may be employed in assisted reproductive technology as a therapeutic effect for this problem. The aim is to assess the level of spermatogenesis and reproductive hormones after PRP autologous intratesticular injection. 50 non-abstructive azoospermia infertile men participated in the current study. The patients were given an intra-testicular injection of platelet-rich plasma using a 1 ml syringe; FSH LH hormonal levels were estimated; if there was no sperm count, the patient should be prepared for a testicular biopsy by the expert doctor. Small number of sperm may be discovered during a biopsy while the testes are being examined in the lab. The therapeutic benefits were seen in 15 patients 3-4 months after using autologous platelet-rich plasma. The results showed that there was a highly significant difference in FSH and LH hormonal levels after PRP injection P(0.001). PRP can help non-obstructive azoospermic infertile males with their testis' structural and functional dysfunction.

References

  1. Kumar R. Medical management of non-obstructive azoospermia. Clinics (Sao Paulo)Feb.2013,68:75-79. Doi:10.6061/clinics/2013 (sup01)08.
  2. Hendriks S, Dancet EA, Meissner A, van der Veen F, Mochtar MH. Perspectives of infertile men on future stem cell treatments for nonobstructive azoospermia. Reprod Biomed Online May 2014, 28 (5):650657.13. Rasha, et al. http://doi.org/10.28969/IJEIR.v12.i1.r7.22 92 Doi: 10.1016/j.rbmo.2014.01.011.
  3. Chang Y, Li J, Chen Y, Wei L, Yang X, et al. Autologous platelet-rich plasma promotes endometrial growth and improves pregnancy outcomes during in vitro fertilization. Int J Clin Exp Med 2015, 8 (1). 1286-1290.
  4. Wang HL, Avila G. Platelet Rich Plasma: Myth or Reality? Eur J Dent 1: Oct. 2007, 1 (4), 192-194.
  5. Pantos K, Nitsos N, Kokkali G, Vaxevanoglou T, Markomichali C, et al. (2016) Ovarian rejuvenation and folliculogenesis reactivation in peri- menopausal women after autologous platelet- rich plasma treatment. Jul. 2016,401. Doi.org/10.26226/morressier.573c1512d462 b80296c98880
  6. Gudelci, M.D., Yigit Cakiroglu, M.D., Aysen Yuceturk, M.D, Bulent Tiras, M.D, Ali Rıza Kural, M.D., Emre Seli, M.D. The effect of intratesticular injection of autologous platelet-rich plasma (PRP) on sperm retrieval rates and IVF outcomes in men with non-obstructive azoospermia and history of failed testicular sperm extraction, Oct. 2021. V (116) p 538. Doi:https://doi.org/10.1016/j.fertnstert.2021.
  7. Gardner DK, Lane M, Watson AJ. A laboratory guide to the mammalian embryo. New York: Oxford University Press; Feb.
  8. Plant TM, Marshall GR. The functional significance of FSH in spermatogenesis and the control of its secretion in male primates. Endocr Rev, Dec. 2001, 22 (6):p764-786. Doi: 10.1210/edrv.22.6.0446.
  9. Kraemer WJ, Rogol AD. The endocrine Rasha, et al. http://doi.org/10.28969/IJEIR.v12.i1.r7.22 93 system in sports and exercise. Malden, Massachusetts: Blackwell Publishing; 2005. no. 30. p 978-1-4051. The Effectiveness of Autologous Platelet-Rich Plasma (PRP) in the Therapy of Infertile Men with Non-Abstractive Azoospermia HSOA Journal of Reproductive Medicine, Gynecology. Sep. 2018, 3 (011). Doi:10.24966/RMGO-2574/100011.
  10. Al-Nasser R, Zakwan Khrait* and Samer,2018, 2004.p.76-85