Cover
Vol. 11 No. 1 (2021)

Published: June 30, 2021

Pages: 13-22

Research Article

Comparison of Results of Semen Parameters Prepared by Swim-up Technique Between the Effect of Adding Autologous Platelet-Rich Plasma and Phosphate Buffer Saline to the Human Semen.

Noor J. Ma'alla 1 Ali I. Rahim 2 Ula M. Al-Kawaz
1High Institute for Infertility Diagnosis and Assisted Reproductive Technologies, Al-Nahrain University, Baghdad, Iraq.
2Faculty of Medicine, University of Kufa, Najaf, Iraq.
History
  • Received: May 25, 2021
  • Accepted: June 23, 2021
  • Available Online: June 30, 2021
DOI

https://doi.org/10.28969/IJEIR.v11.i1.r2.21

Abstract

Swim up is the most popular technique of sperm preparation, in which sperm is selected on motility and ability to swim out the plasma of the semen. The goal of this research is to compare the influence of adding platelet rich plasma (PRP) and phosphate buffer saline in semen prepared by swim – up technique. This research included 119 semen sample evaluated according to sperms parameters stated in WHO and divided into 2 groups. The first group treated with equal quantity of phosphate buffer saline (250) μm contain 10% human serum albumin (HSA) while second group treated with equal quantity of phosphate buffer saline (250) μm contain 10% human serum albumin (HSA) and 2% platelet rich plasma. The samples are prepared by swim – up method. Applying swim up technique using-PBS+PRP (HAS) medium resulted in highest sperm parameters in a highly significant manner (p < 0.001). The values were far better in Swim -up PBS (HAS) with added 2% PRP than those produced by swim up –PBS (HAS) alone. The statistical analysis shows that the variations between Swim up-PBS (HAS) with addition 2% PRP media and swim -up PBS (HAS) were highly significant.

Keywords

References

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