Cover
Vol. 9 No. 2 (2019)

Published: December 31, 2019

Pages: 109-123

Research Article

The Effect of Oxidative Stress on Oocyte Characteristics and Pregnancy Outcome in a Sample of Iraqi Infertile Women Undergoing ICSI

Fatima Abdulrazzaq Al-Muttalibi 1 Hayder A. L. Mossa Liqaa R. Altamimi 2 Wasan A. Abdulhamed
1High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University, Baghdad, Iraq
2Baghdad Specialist Center for Fertility and IVF, Baghdad, Iraq
History
  • Received: October 26, 2019
  • Accepted: July 2, 2020
  • Available Online: November 10, 2020
DOI

https://doi.org/10.28969/IJEIR.v9.i2.r8

Abstract

Oxidative stress in the field of reproduction has been extensively considered in association with sperm properties and male infertility; in any case, the effect of oxidative stress in association with oocyte trademark got less thought than that of sperms. The goal of the study is to understand the possible association among serum and follicular oxidative markers in infertile women and oocyte characteristics and pregnancy result in ICSI cycles. The present case-control study included 57 infertile women undergoing ICSI that was done in the High Institute of Infertility Diagnosis and Assisted Reproductive Technologies at Al-Nahrain University. The assessment started in April 2018 and connected with March 2019. Mean metaphase II oocyte count of all women was 5.70±3.87, that of pregnant women was 6.90±3.68 and that of non-pregnant women was 4.46±3.73; there was a basic qualification in mean metaphase II oocyte check among pregnant and non-pregnant women (P = 0.016); being higher in pregnant women. The mean abnormal oocyte count of all women was 0.63±1.26, that of pregnant women was 0.28±0.45 and that of non-pregnant women was 1.00±1.68. Oxidative stress markers inside and out affected clinical pregnancy result (P < 0.05) and appear to impact clinical pregnancy bring about women undergoing ICSI due to its effect of oocyte characteristics.

Keywords

References

  1. Vander Borght M, Wyns C. Fertility and infertility: definition and epidemiology. Clin Biochem. 2018;62:2–10. Doi: https://doi.org/10.1016/j.clinbiochem. 2018.03.012 [PubMed] [Elsevier].
  2. Elhussein OG, Ahmed MA, Suliman SO, Yahya LI, Adam I. Epidemiology of infertility and characteristics of infertile couples requesting assisted reproduction in a low-resource setting in Africa, Sudan. Fertil Res Pract. 2019;5:7. Doi: https://doi.org/10.1186/s40738-019- 0060-1 [PubMed] [BMC]
  3. Huang JY, Rosenwaks Z. Assisted reproductive techniques. Methods in molecular biology. 2014;1154:171– 231. Doi: https://doi.org/10.1007/978- 1-4939-0659-8_8 [PubMed][Springer Link]
  4. Sharma RS, Saxena R, Singh R. Infertility & assisted reproduction: A historical & modern scientific perspective. Indian J Med Res. 2018;148(Suppl):S10–S14. Doi: https://doi.org/10.4103/ijmr.IJMR_63 6_18 [PubMed] [IJMR]
  5. Oleiwi FD, Mossa HAL, Selman MO. Evaluation of Seminal Plasma TAC in Relevance to Serum DHEA, Testosterone Levels as a Diagnostic Value in Infertile Men. International Journal of Life science and Pharma Research (IJLPR). 2019;9(1):45-51. Doi: http://dx.doi.org/10.22376/ijpbs/lpr.20 19.9.1.L45-51 [IJLPR]
  6. Pizzino G, Irrera N, Cucinotta M, Pallio G, Mannino F, Arcoraci V, Squ adrito F, Altavilla D, Bitto A. Oxidative Stress: Harms and Benefits for Human Health. Oxid Med Cell Longev. 2017; 2017:8416763. Doi: https://doi.org/10.1155/2017/8416763 [PubMed] [Hindawi]
  7. Hindal AS, Mossa HAL, Abood MS. Reactive Oxygen Species Levels in Seminal Plasma in a Sample of Iraqi Infertile Men using Advanced Stimulatory Method for Activation of Spermatozoa. International Journal of Medical Research & Health Sciences. 2018;7(12):51-55. [IJMRHS]
  8. Dutta S, Majzoub A, Agarwal A. Oxidative stress and sperm function: A systematic review on evaluation and management. Arab J Urol. 2019;17(2):87–97. Doi: https://doi.org/10.1080/2090598X.201
  9. Agarwal A, Virk G, Ong C, du Plessis SS. Effect of oxidative stress on male reproduction. World J Mens Health. 2014;32(1):1–17. Doi: https://doi.org/10.5534/wjmh.2014.32. 1.1 [PubMed] [WJMH]
  10. Hussain SS, Al-Murshidi SY, Al- Fatlawi SJ. Association of Endometrial Thickness with Pregnancy Rate in Infertile Women Undergoing ICSI Program. International Journal of Pharmaceutical Research and Allied Sciences. 2018;7(3):166-171. [IJPRAS]
  11. Ahmeid MS. Correlation between follicular fluid leptin and the pregnancy rate in women who underwent ICSI. Tikrit Medical Journal. 2017; 22(1): 248-253. [TMJ]
  12. Fadhil SS, Selman MO, Al-Obaidi MT. Study the Number of Transferred Embryos Increase the Pregnancy Rates in Fresh ICSI-ET-cycles. Global Journal of Bio-science and Biotechnology. 2017;6(4):653-657. [ReseachGate]
  13. Ashrafi M, Jahanian Sadatmahalleh S, Akhoond MR, Ghaffari F, Zolfaghari Z. ICSI Outcome in Infertile Couples with Different Causes of Infertility: A Cross- Sectional Study. Int J Fertil Steril. 2013;7(2):88–95. [PubMed] [IJFS]
  14. Eftekhar M, Mohammadian F, Yousefnejad F, Molaei B, Aflatoonian A. Comparison of conventional IVF versus ICSI in non-male factor, normoresponder patients. Iran J Reprod Med. 2012;10(2):131–136. [PubMed] [IJRM]
  15. Zagadailov P, Hsu A, Seifer DB, Stern JE. Differences in utilization of Intracytoplasmic sperm injection (ICSI) within human services (HHS) regions and metropolitan megaregions in the U.S. Reprod Biol Endocrinol. 2017;15(1):45. Doi: https://doi.org/10.1186/s12958-017- 0263-4 [PubMed] [BMC]
  16. Ozgur K, Bulut H, Berkkanoglu M, Coetzeeb K, Ayc S. Oocyte maturation-index as measure of oocyte cohort quality; a retrospective analysis of 3135 ICSI cycles. Middle East Fertility Society Journal. 2015; 20 (1): 37-42. Doi: https://doi.org/10.1016/j.mefs.2014.04 .005 [Elsevier] [CrossRef]
  17. Hariton E, Kim K, Mumford SL, Palmor M, Bortoletto P, Cardozo ER, Karmon AE, M.D. Sabatini ME, Styer AK. Total number of oocytes and zygotes are predictive of live birth pregnancy in fresh donor oocyte in vitro fertilization cycles. Fertil Steril. 2017;108(2):262–268. Doi: https://doi.org/10.1016/j.fertnstert.201 7.05.021 [PubMed] [Elsevier]
  18. Jozwik Ma, Wolczynski S, Jozwik Mi, Szamatowicz M. Oxidative stress markers in preovulatory follicular fluid in humans. Mol Hum Rep. 1999;5(5):409–413. Doi: https://doi.org/10.1093/molehr/5.5.40 9 [PubMed] [Oxford]
  19. Oral O, Kutlu T, Aksoy E, Fiçicioğlu C, Uslu H, Tuğrul S. The effects of oxidative stress on outcomes of assisted reproductive techniques. J Assist Reprod Genet. 2006;23(2):81– 85. Doi: https://doi.org/10.1007/s10815-005- 9010-4 [PubMed] [CrossRef]
  20. Attaran M, Pasqualotto E, Falcone T, Goldberg JM, Miller KF, Agarwal A, Sharma RK. The effect of follicular fluid reactive oxygen species on the outcome of in vitro fertilization. Int J Fertil Wom Med. 2000;45(5):314– 320. [PubMed]
  21. Agarwal A, Majzoub A. Role of Antioxidants in Assisted Reproductive Techniques. World J Mens Health. 2017;35(2):77–93. Doi: https://doi.org/10.5534/wjmh.2017.35. 2.77 [PubMed] [WJMH]
  22. Agarwal A, Said TM, Bedaiwy MA, Banerjee J, Alvarez JG. Oxidative stress in an assisted reproductive techniques setting. Fertil Steril. 2006;86:503–512. Doi: https://doi.org/10.1016/j.fertnstert.200 6.02.088 [PubMed] [Elsevier]
  23. du Plessis SS, Makker K, Desai NR, Agarwal A. Impact of oxidative stress on IVF. Expet Rev Obstet Gynecol. 2008;3:539–554. Doi: https://doi.org/10.1586/17474108.3.4. 539 [Taylor&Francis]
  24. Agarwal A, Makker K, Sharma R. Clinical relevance of oxidative stress in male factor infertility: an update. Am J Reprod Immunol. 2008;59:2–11. Doi: https://doi.org/10.1111/j.1600- 0897.2007.00559.x [PubMed] [Wiley]
  25. Agarwal A, Majzoub A. Free radicals in andrology. In: Balercia G, Gandini L, Lenzi A, Lombardo F. Antioxidants in andrology. Cham: Springer International Publishing; 2017;1–21. [Springer]
  26. ElSheikh MG, Hosny MB, Elshenoufy A, Elghamrawi H, Fayad A, Abdelrahman S. Combination of vitamin E and clomiphene citrate in treating patients with idiopathic oligoasthenozoospermia: a prospective, randomized trial. Andrology. 2015;3:864–867. Doi: https://doi.org/10.1111/andr.12086 [PubMed] [Wiley]
  27. Galatioto GP, Gravina GL, Angelozzi G, Sacchetti A, Innominato PF, Pace G. May antioxidant therapy improve sperm parameters of men with persistent oligospermia after retrograde embolization for varicocele? World J Urol. 2008;26:97– 102. Doi: https://doi.org/10.1007/s00345-007- 0218-z [PubMed] [SpringerLink]
  28. Peivandi S, Karimpour A, Moslemizadeh N. Effects of L- carnitine on infertile men’s spermogram; a randomized clinical trial. J Reprod Infertil. 2010;10:245– 251. [JRI]
  29. Safarinejad MR. Efficacy of coenzyme Q10 on semen parameters, sperm function and reproductive hormones in infertile men. J Urol. 2009;182:237–248. Doi: https://doi.org/10.1016/j.juro.2009.02. 121 [PubMed] [AUA]
  30. Safarinejad MR, Safarinejad S. Efficacy of selenium and/or N-acetyl- cysteine for improving semen parameters in infertile men: a double- blind, placebo controlled, randomized study. J Urol. 2009;181:741–751. Doi: https://doi.org/10.1016/j.juro.2008.10. 015 [PubMed] [AUA]
  31. Wong WY, Merkus HM, Thomas CM, Menkveld R, Zielhuis GA, Steegers-Theunissen RP. Effects of folic acid and zinc sulfate on male factor subfertility: a double-blind, randomized, placebo-controlled trial. Fertil Steril. 2002;77:491–498. Doi: https://doi.org/10.1016/S0015- 0282(01)03229-0 [PubMed] [Elsevier]
  32. Omu AE, Al-Azemi MK, Kehinde EO, Anim JT, Oriowo MA, Mathew TC. Indications of the mechanisms involved in improved sperm parameters by zinc therapy. Med Princ Pract. 2008;17:108–116. Doi: https://doi.org/10.1159/000112963 [PubMed] [Krager]
  33. Lenzi A, Sgrò P, Salacone P, Paoli D, Gilio B, Lombardo F. A placebo- controlled double-blind randomized trial of the use of combined l-carnitine and l-acetyl-carnitine treatment in men with asthenozoospermia. Fertil Steril. 2004;81:1578–1584. Doi: https://doi.org/10.1016/j.fertnstert.200 3.10.034 [PubMed] [Elsevier]
  34. Ciftci H, Verit A, Savas M, Yeni E, Erel O. Effects of N-acetylcysteine on semen parameters and oxidative/antioxidant status. Urology. 2009;74:73–76. Doi: https://doi.org/10.1016/j.urology.2009 .02.034 [PubMed] [Elsevier]
  35. Greco E, Iacobelli M, Rienzi L, Ubaldi F, Ferrero S, Tesarik J. Reduction of the incidence of sperm DNA fragmentation by oral antioxidant treatment. J Androl. 2005;26:349–353. Doi: https://doi.org/10.2164/jandrol.04146 [PubMed] [Wiley]
  36. Raheem FA, Mossa HAL, Abdulhamed WA, Altamimi LR. Assessment of Oxidative Stress Changes in Serum and Follicular Fluid in Relevance to GnRH Rival Protocol in Iraqi Infertile Ladies Undergoing ICSI. European Journal of Medical and Health Sciences. 2019;1(5):1-5. Doi: https://doi.org/10.24018/ejmed.2019.1 .5.121 [EJMHS] Biography Dr. Fatima Abdulrazzaq Al- Muttalibi She graduated from Almustansiryia University, college of medicine in 2008. She started her permanency in Obstetrics and Gynecology Department at Alzahra’ teaching hospital, Wasit, Iraq in 2015. She got accepted at the High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University to study clinical infertility in 2017. Currently, she is about to finish the degree to get a High Diploma degree in clinical infertility and ART. Dr. Hayder A. L. Mossa He is an assistant professor at Al Nahrain University. He is a member of the Iraqi Society of Fertility, Iraqi Society of Zoonotic Diseases (ISZD) & Iraqi Zoonotic Communicable Diseases (ISZCD), Iraqi Academics Syndicate, European Society of Human Reproduction and Embryology (ESHRE). An editorial board member of Merit Research Journal of Medicine and Medical Sciences (MRJMMS), International Journal of Medical Research & Health Sciences (IJMRHS). A reviewer at global journals Inc. (USA). He was a candidate for the award of Arabian Young Researchers in 2007. He granted for many appreciation certificates in the scientific & administrative fields. He participated & supervised in more than 60 scientific & medical conferences, workshops & training course. Has many kinds of research & articles published in Iraqi & national medical journals. Chief & member of nearly 60 scientific & administrative committees from 2006-till now. Has interests in assisted reproductive technologies, reproductive micromanipulation, medical terminology, research methods and seminars, reproductive physiology, advanced embryology. Dr. Liqaa R. Altamimi She is a Senior Clinical Embryologist, the director of Baghdad Specialized IVF and Genetics Center in Baghdad. She got B.Sc. in Medical Microbiology from Baghdad and the High Diploma in Medical Laboratory Analysis in Baghdad. She got her Ph.D. in Genetic Engineering from Charles Molnar University in Malaysia 2012. She published many kinds of research in global journals in Clinical Embryology, Reproductive Medicine during the last 15 years. She is the Pioneer Clinical Embryologist, many Embryologists had been practiced under her marvelous supervision. She is a member of the European Society of Human Reproduction And Embryology (ESHRE), American Society of Reproductive Medicine (ASRM), Middle East Fertility Society (MEFS), and an active member in Iraqi Fertility Society (IFS). She had multiple certificates for training and supervising from many centers worldwide. Dr. Wasan A. Abdulhameed She received M.B.CH.B. from the University of Baghdad, College of medicine in 1992, the diploma and the Arab Board in Gyn.&Obs from Medical City, Baghdad in 1998. She worked in Al Sharjah University, UAE. She earned the laparoscopic surgery certificate from India in 2009. She was a Lactation consultant in Australia in 2010. She occupied many administrative positions She attended more than 20 academic congresses internationally. How to cite: Al-Muttalibi FA, Mossa HAL, Altamimi LR, Abdulhameed WA. The Effect of Oxidative Stress on Oocyte Characteristics and Pregnancy Outcome in a Sample of Iraqi Infertile Women Undergoing ICSI; Iraqi Journal of Embryos and Infertility Researches (IJEIR), (2019); 9(2): 109-123. Doi: http://doi.org/10.28969/IJEIR.v9.i2.r8 © 2019 Author(s)