Cover
Vol. 9 No. 1 (2019)

Published: November 30, 2019

Pages: 15-31

Research Article

The New Approach of Hyaluronic Binding Assay in Relevance to Sperm Activation by Direct Swim-up Technique in Iraqi Infertile Men

Ezdehar N. Ali 1 Hayder A. L. Mossa Ula M. R. Al-Kawaz
1High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al Nahrain University, Baghdad, Iraq
History
  • Received: May 26, 2019
  • Accepted: November 1, 2019
  • Available Online: November 14, 2019
DOI

https://doi.org/10.28969/IJEIR.v9.i1.r2

Abstract

Hyaluronic acid (HA) is a polysaccharide, that composed in the extracellular matrix of the cumulus cells, and considered to take function in choosing mature spermatozoa. The Sperm-hyaluronic binding assay (HBA) is significant diagnostic instrument for suspected male infertility when examining the semen. The HBA slide supplies the ratio of mature binding sperm in the specimen. To evaluate sperm parameters, and to evaluate the (HBA Score percentage (%) before and after activation. Twenty-five infertile couples a sharer in this current study through their attendance to the Al Nahrain University. Males undergoing seminal fluid analysis were done according to (WHO 2010, and WHO 1999). Sperm parameters assessed and calculate the HBA Score% pre-and-post-direct swim-up technique, the results were statistically tested. In Post-activation there was considerable increment noticed in the progressive motility and morphology of spermatozoa, likewise significant improvement in the (HBA score %). Furthermore, considerable reduction in the concentration, agglutination of spermatozoa and round cells count, when compared to pre-activation. The mean of binding of spermatozoa to hyaluronan pre-activation (direct swim-up technique) was less than normal limit for normozoospermic males. However, it significantly improves post-activation.

Keywords

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  34. Nixon B, Aitken RJ, McLaughlin EA. New insights into the molecular mechanisms of sperm-egg interaction. Cellular and Molecular Life Sciences. Springer Science and Business Media LLC; 2007, 20;64(14):1805–23. Doi: http://dx.doi.org/10.1007/s00018 -007-6552-x [PubMed][Springer] Biography Dr. Ezdehar N. Ali She was born in Thi-Qar in 1969. She received the M.B.Ch.B.- College of Medicine - University of Basra – 1992, the D.G.O.- College of Medicine - University of Basra 2004, the M.Sc. in Applied Embryology the High Institute of Infertility Diagnosis and Assisted Reproductive Technologies, Al-Nahrain University, in 2015. She worked as a gynecologist and obstetrician in 2004-2013. She is a Member of the Iraqi Society of Fertility. Currently she is a lecturer in the College of Medicine at the University of Thi- Qar - Department of Obstetrics and Gynecology. Dr. Hayder A. L. Mossa He is an assistant professor at Al Nahrain University. He is a member of the Iraqi Society of Fertility, Iraqi Society of Zoonotic Diseases (ISZD) & Iraqi Zoonotic Communicable Diseases (ISZCD), Iraqi Academics Syndicate, European Society of Human Reproduction and Embryology (ESHRE). An editorial board member of Merit Research Journal of Medicine and Medical Sciences (MRJMMS), International Journal of Medical Research & Health Sciences (IJMRHS). A reviewer at global journals Inc. (USA). He was a candidate for the award of Arabian Young Researchers in 2007. He granted for many appreciation certificates in the scientific & administrative fields. He participated & supervised in more than 60 scientific & medical conferences, workshops & training course. Has many kinds of research & articles published in Iraqi & national medical journals. Chief & member of nearly 60 scientific & administrative committees from 2006-till now. Has interests in assisted reproductive technologies, reproductive micromanipulation, medical terminology, research methods and seminars, reproductive physiology, advanced embryology. Dr. Ula M. R. Al-Kawaz He is a Professor of Urology at Al-Nahrain University. Currently he is the dean of the high institute of infertility diagnosis and ARTs, Al- Nahrain University. He is a fellow of the Iraqi Board for Medical Specialization, Council of Urology since 2006, and a fellow of the European Board of Urology, since 2009. He published more than 27 articles both local and international. He supervised many M.Sc. and Ph.D. students. How to cite: Ali EN, Mossa HAL, Al-Kawaz UMR. The New Approach of Hyaluronic Binding Assay in Relevance to Sperm Activation by Direct Swim-up Technique in Iraqi Infertile Men; Iraqi Journal of Embryos and Infertility Researches (IJEIR), (2019); 9(1):15-31. Doi: http://doi.org/10.28969/IJEIR.v9.i1.r2 © 2019 Author(s)